本研究利用微卫星标记技术从分子水平对云南5个地方绵羊品种(腾冲绵羊(TC)、昭通绵羊(ZS)、迪庆绵羊(DQ)、宁蒗黑绵羊(NL)、乌骨绵羊(WG))进行了分析,通过计算平均杂合度、多态信息含量等对它们之间的亲缘关系及群体间、群体内的遗传变异进行了探讨。结果发现,各品种内杂合度较低,在0.2894-0.3349之间,说明各群体内遗传变异较小;PIC值均大于0.5,呈现高度多态,说明5个绵羊品种具有较丰富的遗传多样性。5个绵羊品种之间的基因流分析结果发现,除与乌骨绵羊之间的基因交流较小外,其他4个绵羊品种之间的基因交流都较大。应用UPGMA法进行系统发生关系分析,乌骨绵羊同其余品种亲缘关系较远,最近的是迪庆绵羊和宁蒗黑绵羊。
Five local sheep breeds of Tengchong (TC), Zhaotong sheep (ZS), Diqing (DQ), Ninglang (NL) and Wugu (WG) in Yunnan were analyzed through the microsatellite DNA marker technology. The genetic variability within breeds and genetic relationship among breeds, the genetic heterozygosity (H), polymorphism information content (PIC), coefficient of gene differentiation and genetic distance etc had been evaluated. The results showed that the heterozygosity of five sheep breeds were 0.2894 to 0.3349, this indicated that genetic variability was low in analyzed sheep. The PIC value was high, beyond 0.5, it indicated the five local sheep breeds in Yunnan had more abundant genetic diversity. The gene flow had been analyzed, there were bigger gene flow within sheep breeds except for WG sheep. The Dcoa genetic distance and Ds genetic distance had been analyzed by using POPGENE software, and drawed the dendrograms with the above genetic distance, the genetic distance between WG sheep and other sheep were far, the close were between Diqing sheep and Ninglang sheep.