中文摘要：

[目的]测定海洋放线菌S.areniocola CNS-205腺苷化结构域基因sare0357的酶活.[方法]选取海洋放线菌Salinispora arenicola CNS-205 NRPS A domain基因sare0357,对其进行克隆表达和功能鉴定.[结果]在以Trp为底物时测定Sare0357蛋白的酶动力学参数为：Km=（0.040 33±0.003 67） mmol/L,Vmax=（2.135 05±0.029 43） μmol/（min·L）,kcat=（14.233 6±0.196 2）min;Phe为底物时：Km=（0.027 65±0.001 51） mmol/L,Vmax=（1.55806±0.009 7）μmol/（min·L）,kcat=（10.387 1±0.064 6）min.[结论]丰富洋放线菌Salin-ispora arenicola CNS-205 NRPS腺苷化结构域的底物特异性和密码子领域的研究,为组合生物学和体外酶系合成NRPs提供依据.

英文摘要：

[Objective] The aim was to determine enzyme activity of sare0357,a gene of adenylation domain from marine actinomycete S.arenicola CNS-205.[Method] Marine actinomycetes Salinispora arenicola CNS-205 NRPS A domain gene sare0357 was selected,and the cloning expression and function identification were done.[Result] It was found that Sare0357 could recognize specifically and activate tryptophan （Try） and phenylalanine （Phe）.The basic kinetic parameters of it for these two substrates were detected as Km =（0.040 33 ± 0.003 67） and （0.027 65 ±0.001 51）mmol/L,Vmax =（2.13505 ±0.02943） and （1.55806 ±0.009 7） μmol/min and kcat =（14.233 6 ± 0.196 2） and （10.387 1 ±0.064 6） min for Try and Phe respectively.[Conclusion] The aim riched ocean actinomycetes Salinispora arenicola CNS-205 NRPS of adenosine structure domain research in the field of the substrate specificity and codes,and provided the basis for combination of biology and enzyme system in vitro synthetic NRPS.