中文摘要：

目的探讨脂多糖（LPS）、肿瘤坏死因子α（TNFα）对大鼠肺微血管内皮细胞（RPMVEC）内游离钙浓度（[Ca^2＋]i）的影响。方法将细胞接种于盖玻片上，细胞单层汇合后进行实验。实验分组：LPS组（用10ug/mlLPS），LPS＋山莨菪碱组（用10ug／mlLPS＋10ug/ml山莨菪碱），TNFα组（用5000u／mlTNFα），TNFα＋山莨菪碱组（用5000u／mlTNFα＋10ug／ml山莨菪碱），正常对照组加等量稀释液。作用15、90min后按照试剂盒提供的方法，并参照文献测定[Ca^2＋]i。结果与正常对照组比较，LPS组、LPS＋山莨菪碱组15min和90min两个时相点[Ca^2＋]i均显著升高（P〈0．01）；LPS组与LPS＋山莨菪碱组比较，15min和90min两个时相点[Ca^2＋]无明显差异（P〉0．05）。同样，与正常对照组比较，TNFα组、TNFα＋山莨菪碱组15min和90min两个时相点[Ca^2＋]i均显著升高（P〈0．01）；TNFα组与TNFα＋山莨菪碱组比较，15min和90min两个时相点[Ca^2＋]i无明显差异（P〉0．05）。结论LPS、TNFα作用可使RPMVEC[Ca^2＋]i显著升高，山莨菪碱对LPS、TNFα诱导的RPMVEC[Ca^2＋]i升高无显著抑制作用；LPS、TNFα引起的[Ca^2＋]i浓度升高与β—AR介导的信号转导通路无关；[Ca^2＋]i升高可能参与了G蛋白偶联受激酶的活性调节。

英文摘要：

Objective To investigate the effects of lipopolysaecharide （LPS） and tumor necrosis factor α （ TNF α ） on intracellular free cal- cium （ [ Ca^2 ＋ ] i ） of rat pulmonary mierovaseular endothelial cells （ RPMVEC ） and the relationship between [ Ca^2 ＋ ] i. and beta adrenoeeptor （ β-AR ）. Methods Equal amount of cells was incubated on glass films ; experiment was carried out when the cell monolayer confluented; group division in the experiment was as follows： LSP group （ 10ug/ml of LPS added） , LPS ＋ anisodamine group （ 10ug/ml of LPS and 10ug/ml of anisodamine added） , TNFα group （5000u/ml of TNFα added） , TNFα ＋ anisedamine group （5000u/ml of TNFα and 10ug/ ml of anisodamine added） and normal control group （ equal amount of diluent added ） , and each group had 4 samples. [ Ca^2 ＋ ] i. In each group was measured at the time point of 15 and 90 minute with the method from reagent kit instruction and literature. Result [ Ca^2＋ ] i in LPS group and LPS ＋ anisodamine group at the time point of 15 and 90 minutes were all significantly higher than that in normal control group （P 〈 0.01 ）, but there existed no significant difference of [ Ca^2＋ ] i between LPS group and LPS ＋ anisodamine group at the same time points （ P 〈 0.01 ） ; [ Ca^2 ＋ ] i in TNFα group and TNFα ＋ aaisodamine group at the time point of 15 and 90 minute were also significantly higher than that in normal control group （ P 〈 0.01 ） but there was no significant difference of [ Ca^2＋ ] i. between TNFα group and TNFα ＋ anisodamine group at the time point of 15 and 90 minute. Conclusions Both LPS and TNFα can lead to the significant increase of [ Ca^2＋ ] i in RPMVEC ; the increase of [ Ca^2＋ ] i is not related to the signal transduction pathway mediated by beta adrenoceptor while [ Ca^2＋ ] i can participated in the regulation of G protein coupled receptor kinases.