中文摘要：

以紫扇贝DNA为模板,用已开发的147个海湾扇贝微卫星标记引物扩增,结果表明100个微卫星标记能成功扩增,其中有47个表现出多态性,等位基因数目从2到9不等.观测杂合度范围为0.128 0～1.000 0（平均0.660 4）,期望杂合度范围为0.503 1～0.862 1（平均0.671 9）,有6个位点偏离Hardy-Weinberg平衡（P〈0.05）.用7对引物分别对紫扇贝、海湾扇贝及其种间正反杂交F1各30个个体进行PCR扩增,发现它们可明确区分紫扇贝与海湾扇贝,且所检测60个杂交子代均同时含海湾扇贝与紫扇贝的相应种特异性条带,证明全部为种间杂交子代.将该7对引物的扩增产物克隆测序,发现这些位点在两种扇贝中的序列同源率为40.22%～91.95%,其中3个位点在紫扇贝中的扩增产物仍然含有微卫星.

英文摘要：

In order to investigate transferability of microsatellite markers in genus Argopecten,147 mi-crosatellite markers developed from the bay scallop（A.irradians）,were amplified in 30 Peruvian scallop（A.purpuratus） individuals.One hundred loci can be amplified in the Peruvian scallop,of which 47 loci were found to be polymorphic with the number of alleles per locus ranging from 2 to 9.The observed heterozygos-ity（Ho） of these loci ranged from 0.128 0 to 1.000 0（with an average of 0.660 4） while the expected het-erozygosity（He） ranged from 0.503 1 to 0.862 1（with an average of 0.671 9）.Six loci were found to be sig-nificantly departed from Hardy-Weinberg Equilibrium.Seven loci were selected for progeny identification in the Peruvian scallops,the bay scallops and their hybrids.The results showed that these SSR（simple se-quence repeat） markers are able to distinguish between the Peruvian and the bay scallops.Amplification with these SSR markers in 60 hybrids displayed bands from both parents,indicating that these animals were true hybrids.Sequencing of these 7 loci in the Peruvian scallop showed they have 40.22%～91.95% identity with their counterparts in the bay scallop and 3 of them contained SSRs.Development of these markers will provide necessary and powerful molecular markers in selective and hybridization breeding involving the bay scallop and the Peruvian scallop in the future.