中文摘要：

目的探讨氟西汀(fluoxetine,FLXT)对创伤后应激障碍（post-traumatic-stress-disorder，PTSD）大鼠记忆及大鼠海马神经元突触后致密物蛋白95（postsynaptic density95,PSD-95）和突触素I（synapsinⅠ）表达的影响。方法采用国际认定的SPS方法刺激大鼠建立PTSD大鼠模型，应用水迷宫实验观察氟西汀对PTSD大鼠学习记忆的影响，采用免疫荧光染色和免疫印迹法检测海马神经元PSD-95和突触素I水平。结果Morris水迷宫前5天的定位航行实验结果显示，SPS刺激后大鼠（PTSD大鼠）找到水下平台的游泳距离和潜伏期比正常组大鼠长，给予氟西汀的PTSD大鼠找到水下平台的距离和潜伏期较未用氟西汀处理的PTSD大鼠缩短。Morris水迷宫第6天空间探索实验结果显示，PTSD大鼠穿越平台的次数和在靶象限花费时间的百分比明显低于正常对照大鼠，而氟西汀可显著增加PTSD大鼠在水迷宫实验中的穿台次数和在靶象限停留的时间百分比。免疫荧光染色和WesternBlot检测显示，PTSD大鼠PSD-95和突触素I的水平降低，氟西汀干预可抑制PTSD大鼠PSD-95和突触素Ⅰ水平的降低。结论氟西汀可通过抑制PTSD大鼠海马神经元细胞PSD-95和SynapsinⅠ水平的下调，减轻PTSD大鼠空间记忆和学习能力的损伤。

英文摘要：

Objective To investigate the effect of fluoxetine on the memory post-traumatic-stress-disorder(PTSD)rats and theexpression of postsynaptic density95(PSD-95)and synapsinⅠin the hippocampal neurons of PTSD rats.Methods The PTSD rat modelwas established by the internationally recognized SPS method.Morris water maze(MWM)experiment was performed to observe theeffect of fluoxetine on the learning ability and memory of PTSD rats.The expression of PSD-95and synapsinⅠin hippocampal neuronswas detected by immunofluoresent staining and western blot.Reults In the positioning navigation test during the first five days ofMWM experiment,the swimming distance and latency taken by the PTSD rats to find the underwater platform were longer than thosetaken by normal rats or PTSD rats treated with fluoxetine;on the sixth day of MWM experiment in the space exploration test,the timesthe rats crossed the platform and the percentage of time spent in the target quadrant were obviously lower in the PRSD group than inthe normal group,and fluoxetine treatment significantly increased these two indexes.Immunofluoresent staining and western blot resultsshowed that the levels of PSD-95and synapsinⅠdecreased in the PTSD rats and that fluoxetine treatment could inhibit this effect.Conclusion Fluoxetine can relieve the impairment of the special memory and learning ability of PTSD rats by inhibiting the decreaseof the levels of PSD-95and synapsinⅠin the hippocampal neurons of the rats.