中文摘要：

目的：寻找一种简单、快速、更有效的检测心脏细胞增殖的方法,以探讨损伤心脏再生潜能的机制。方法：取小鼠不同时期（胚胎、新生及成年期）及不同生理状态下（健康、心肌梗死后）心脏行组织冰冻切片后,用免疫荧光染色法分别标记心脏细胞：心肌细胞（心脏特异转录因子Nkx 2.5和Gata 4,心肌特异性标记蛋白TnT）,内皮细胞（CD34）,血管平滑肌细胞（SMMIgG）,心脏干细胞（IsL1,Tbx18和Wt1）;在此基础上用EdU染色法检测不同心脏细胞的增殖能力。结果：EdU能强烈地标记不同时期及不同生理状态下小鼠心脏细胞的增殖;心梗后心脏细胞进入细胞增殖周期,但增殖能力非常有限。结论：EDU可简单、快速、更有效的检测心脏细胞增殖,为探讨损伤心脏再生潜能机制提供了新的方法。

英文摘要：

Objective：To find a rapid and sensitive method in identificating cardiac cellular proliferation in order to understand the underlying mechanisms and strategies of cardiac regeneration.Methods：The following immunologically related markers of cardiac cells were analyzed：cardiac transcription factors Nkx 2.5and Gata 4;specific marker of cardiomyocytes TnT;endothelial cell marker CD34;vascular smooth muscle marker smooth muscle myosin IgG（SMM）;cardiac resident stem cells markers IsL1,Tbx18,and Wt1.Results：Markers were co-localized in cardiac tissues of embryonic,neonatal,adult,and pathological samples by 5-ethynyl-2＇-deoxyuridine（EdU）staining.EdU was also used to label isolated neonatal cardiomyocytes in vitro.EdU robustly labeled proliferating cells in vitro and in vivo,co-immunostaining with different cardiac cells markers.Conclusion：EdU can rapidly and sensitively label proliferating cardiac cells in developmental and pathological states.Cardiac cell proliferation assessed by EdU is a novel analytical tool for investigating the mechanism and strategies of cardiac regeneration in response to injury.