中文摘要：

目的：探讨绿原酸对缺氧复氧导致的心肌细胞内锚定蛋白-B（ankyrin-B）下降的调节作用及其机制。方法：培养乳鼠心肌细胞,分为对照组、缺氧复氧组和绿原酸处理组。以流式细胞法检测心肌细胞内活性氧（ROS）的水平,用Western-blot检测心肌细胞内ankyrin-B的表达。结果：缺氧复氧后心肌细胞内ROS水平明显增高,绿原酸预处理能够抑制ROS水平的增高[对照组：（237.6±22.3）;缺氧复氧组：（612.1±25.6）,绿原酸处理组：（375.9±30.1）;P〈0.05]。缺氧复氧后心肌细胞内ankyrin-B的表达显著降低,绿原酸预处理能够上调ankyrin-B的表达。结论：绿原酸可能通过清除细胞内的ROS从而减少缺氧复氧引起的心肌细胞内ankyrin-B的表达降低。

英文摘要：

Objective： To investigate the effect and the mechanism of chlorogenic acid on the reduction of ankyrin-B induced by hypoxia/reoxygenation in cardiomyocytes. Method： Cultured cardiomyocytes of neonatal SD rats had been divided into control, hypoxia/reoxygenation （H/R） and hypoxia/reoxygenation ＋ Chlorogenic acid （H/R＋CGA） group. The level of reactive oxygen species（ROS）was detected by flow cytometry and the expres- sion of ankyrin-B in cardiomyocytes was detected by western-blot. Result：The level of ROS was significantly in- creased by H/R and the increasing was decreased by pretreatment with chlorogenic acid [control group.. （237.6±22.3）, H/R group： （612.1±25.6）,H/R＋CGA group： （375.92=30.1） ;P〈0.05]. The expression of ankyrin-B in cardiomyocytes was significiantly decreased by H/R and was increased by pretreatment with chlorogenic acid. Conclusion： Chlorogenic acid was able to prevent cardiomyocytes injury induced by H/R by regulating ankyrin-B.