中文摘要：

目的调查青海省大骨节病病区和非病区儿童血清钙、磷、铁、锌和碱性磷酸酶等生化指标现状。方法根据青海省大骨节病监测结果，选择青海省兴海县唐乃亥乡和化隆县甘都镇作为大骨节病病区，以兴海县曲什安乡作为非病区，于2014年6月分别在上述地区寄宿小学中选择7～15岁儿童作为调查对象．采集血样，采用酶比色法检测血清碱性磷酸酶含量，比色法检测血清铁、锌、钙离子和无机磷含量。并计算钙磷比值。结果在青海省病区和非病区各采集合格儿童血样59、45份，血清碱性磷酸酶含量分别为（311．34±85．31）、（264．09±73．44）U／Ｌ，二者比较差异有统计学意义（t=－2．97，P〈0．05）；儿童血清磷含量分别为（1．62±0．17）、（1．43±0．13）mmol／L，二者比较差异有统计学意义（t=－6．29，P〈0．05）；钙磷比值分别为1．62±0．17、1．82±0．21，二者比较差异有统计学意义（t=5．53，P〈0．05）；儿童血清锌含量分别为（19．96±1．70）、（20．59±2．45）μmol／L，血清钙含量分别为（2．59±0．11）、（2．57±0．11）mmol／L，血清铁含量分别为（15．06±7．02）、（17．01±6．70）μmol／L，病区与非病区儿童血清锌、钙、铁含量比较，差异无统计学意义（￡=1．39、－0．64、1．44，P均〉０．05）。结论青海省大骨节病病区儿童血清碱性磷酸酶和无机磷水平显著高于非病区，钙磷比值显著降低。

英文摘要：

Objective To investigate the serum levels of biochemical indexes such as calcium, phosphorus, iron, zinc, and alkaline phosphatase of children in Kasehin-Beck disease areas and control areas in Qinghai Province. Methods According tot he results of Kasehin-Beck disease monitoring in Qinghai Province, Tangnaihai Township of Xinghai County and Gandu Town of Hualong County were chosen as Kaschin-Beck disease areas and Qushian Township of Xinghai Country was chosen as a control. Children aged 7 to 15 in boarding schools in these areas were Chosen as the study subjects in June 2014. Serum alkaline phosphatase level was determined by enzyme colorimetry and the serum iron, zinc, calcium and phosphorus levels were determined by colorimetry. Calcium to phosphate ratio was calculated at the same time. Results Fifty-nine qualified serum samples of children were sampled in Kaschin-Beck disease areas and 45 in control area. Serum alkaline phosphatase levels in Kaschin-Beck disease areas and control area were （311.34 ± 85.31） and （264.09± 73.44）U/L, respectively, and the difference was statistically significant （t = - 2.97, P 〈 0.05）. Children＇s serum phosphorus levels in Kaschin-Beck disease areas and control area were （1.62 ± 0.17） and （1.43 ± 0.13）mmol/L, respectively, and the difference was statistically significant （t = - 6.29, P 〈 0.05）. Calcium to phosphorus ratio were 1.62 ± 0.17 and 1.82 ± 0.21, respectively, and the difference was statistically significant （t = 5.53, P 〈 0.05）. Compared with the control area, the level of children＇s serum zinc was （19.96 ± 1.70） and （20.59 ±2.45）μmol/L; the level of serum calcium was （2.59± 0.11） and （2.57 ±0.11）mmol/L; and the level of serum iron was （15.06 ± 7.02） and （17.01 ± 6.70）μmol/L. The differences between these three biochemical indicators were not statistically significant between Kaschin-Beck disease areas and control area （t = 1.39, - 0.64, 1.44, all P 〉 0.05）. Conclusion In Kaschin-Beck dis