中文摘要：

研究了玉米芯的酶法水解及酶解液的乙醇发酵。采用里氏木霉ZU-02纤维素酶水解酸预处理后的玉米芯为原料，适宜的酶用量为20 FPIU（以每克底物计，下同），48h后酶解得率为67．5％；添加黑曲霉ZU-07所产纤维二糖酶可有效解除纤维二糖累积引起的反馈抑制作用，当纤维二糖酶用量为6．5 CBIU时，48h后酶解得率提高到83.9％。采用分批补料酶解工艺，使底物质量浓度提高到200g/L，酶解60h后还原糖质量浓度达到116．3g/L，酶解得率为80．1％。利用一株耐高温酿酒酵母HTR-11在38℃下时酶解液进行乙醇发酵，质量浓度95．3g/L的葡萄糖在18h内发酵生成质量浓度为45．7g/L的乙醇，其得率达到理论值的94％。

英文摘要：

Enzymatic hydrolysis of corncob for ethanol fermentation from cellulosic hydrolysate was investigated. Corncob pretreated with dilute sulfuric acid was hydrolyzed by the cellulase from Trichoderma reesei ZU-02 at 50 ℃, pH value 4.8, and the hydrolysis yield was only 67.5 % due to poor cellobiase activity in T. reesei ZU-02 cellulase. Addition of cellobiase from Aspergillus niger ZU-07 greatly reduced the inhibitory effect caused by cellobiose, and the hydrolysis yield was improved to 83.9 % with cellobiase dosage of 6.5 CBIU/g substrate. A hydrolysis process using batch-wise feed was carried out to get a final substrate concentration of 200 g/L and the hydrolysis yield reached 80. 1 % after 60 h reaction. Furthermore, ethanol fermentation of cellulosic hydrolysate containing 95.3 g/L glucose was performed at 38 ℃ using a thermotolerant strain Saccharomyces cerevisiae HTR-11,45.7 g/L ethanol was obtained within 18 h and ethanol yield reached 94 % of the theoretical yield.