中文摘要：

目的 研究microRNA-9在卵巢癌细胞侵袭与转移中的作用,探讨microRNA-9与E-cadherin及snail1之间的关系。方法 在RNA水平上通过RT-PCR方法验证转染miR-9 mimics和inhibitor后对上皮指标E-cadherin以及转录因子snail1的影响;在蛋白水平上通过western blot方法验证转染miR-9 mimics和inhibitor后对E-cadherin及sanil1表达的影响;划痕实验及transwell实验验证转染miR-9 mimics和inhibitor后对卵巢癌细胞侵袭与转移能力的影响。结果 RT-PCR结果表明转染miR-9 mimics后E-cadherin明显下调,snail1明显上调,反之转染miR-9 inhibitor后E-cadherin明显上调,snail1明显下调;western blot证实转染miR-9 mimics后E-cadherin表达明显下调,snail1明显上调,反之转染miR-9 inhibitor后E-cadherin明显上调,snail1明显下调;划痕实验表明转染miR-9后卵巢癌细胞愈合能力增强,transwell结果表明转染miR-9 mimics后卵巢癌细胞转移能力明显增强,转染miR-9 inhibitor后转移能力下降。结论 在卵巢癌细胞中miR-9可能通过调控E-cadherin/snail1影响卵巢癌细胞侵袭与转移能力。

英文摘要：

Objective To study the role of microRNA- 9 in invasion and migration of ovarian cancer cells,explore the relationship between microRNA- 9 and E- cadherin / snail1. Methods After transfecting miR- 9 mimics or inhibitor into SKOV3 cells,RT- PCR was performed to confirm the effect on epithelial marker E- cadherin as well as transcript factor snail1 at RNA level; western blot was performed to confirm the effects on E- cadherin and snail1 at protein level; wound healing assay and transwell chamber were used to confirm the effect on invasion and migration capability of ovarian cancer cells. Results RT- PCR demonstrated that E- cadherin dramatically decreased and snail1 greatly increased at mRNA level in SKOV3 cells transfected with miR- 9 mimics; while after treated with miR- 9 inhibitor,SKOV3 cells expressed higher level of E- cadherin and lower level of snail1. The same results were also illustrated at protein level through western blot. Wound healing assay suggested that SKOV3 cells displayed better healing ability after miR- 9 transfection. Transwell chamber migration assay showed significantly increased cell migration ability after miR- 9 mimics transfection and decreased cell migration ability after miR- 9 inhibitor transfection. Conclusion miR- 9 may impact invasion and migration abilities of ovarian cancer cells via regulating E- cadherin / snail1 expressions.