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玉米秸秆低温降解复合菌系的筛选
  • ISSN号:0578-1752
  • 期刊名称:《中国农业科学》
  • 时间:0
  • 分类:S154.3[农业科学—土壤学;农业科学—农业基础科学]
  • 作者机构:[1]内蒙古农业大学农学院,呼和浩特010019
  • 相关基金:国家科技支撑计划(2011BAD16B13,2012BAD04B04,2013BAD07B04,2011BAD16B14)、国家玉米产业技术体系(CARS-02-63)、国家自然科学基金(31260300)
中文摘要:

【目的】为加快北方高寒地区玉米秸秆降解速度,筛选低温条件下具有良好降解玉米秸秆的复合菌系。【方法】以富含纤维素的腐烂物为菌源,通过滤纸崩解初筛、酶活和秸秆降解率为指标复筛进行玉米秸秆降解菌系的筛选,并对其成分进行分析。【结果】从腐烂的树叶和高原锯末中筛选到两组玉米秸秆降解复合菌系1号和8号。1号和8号复合菌系在玉米秸秆培养基中,15℃培养15 d,玉米秸秆分解率分别达到30.21%和32.21%;1号复合菌系包含木霉和多种细菌,8号复合菌系含青霉和多种细菌;两组复合菌系优势细菌均为梭菌属和芽孢杆菌属菌种。【结论】筛选到的菌系在低温(15℃)实验室条件下能降解玉米秸秆,菌系主要组成是木霉、青霉、梭菌属(Clostridium sp.)、芽孢杆菌属(Bacillus sp.)和草螺菌属(Herbaspirillum sp.)的细菌。

英文摘要:

【Objective】 For the purpose of accelerating the northern region maize straw degradation speed, a research was conducted to screen microorganism which has a good ability to degradate maize straw under the conditions of low temperature. 【Method】 In this experiment, materials rich in cellulose were used as bacteria sources, through preliminary screening by filter-paper disintegration indicator, enzyme activity and straw degradation rate re-screening, the maize straw degradation strains were selected, then its composition was analyzed. 【Result】 Using this method, two cellulose-degrading microflora (designated as No.1 and No.8) capable of effectively degrading maize straw was enriched from plateau rotted sawdust successfully. The microflora No.1 and No.8 were inoculated in the medium with maize straw as a sole carbon and energy source. The degradation rate of maize straw were 30.21% and 32.21% after 15 days cultivation under 15℃. Two groups of composite microbial systems were composed of fungi and bacteria.No.1 strains of fungus were Trichoderma, and No.8 strains fungus were Penicillium. Two groups of composite microbial system was mainly composed of Clostridium bacteria, Bacillus and oxalic acid bacillus bacteria. 【Conclusion】 Screening of the strains under low temperature (15℃) could degrade the maize straw under laboratory conditions, and the microbial system was mainly comprised of Trichoderma, Penicillium, Clostridium, Bacillus and Herbaspirillum.

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期刊信息
  • 《中国农业科学》
  • 中国科技核心期刊
  • 主管单位:中华人民共和国农业部
  • 主办单位:中国农业科学院 中国农学会
  • 主编:万建民
  • 地址:北京中关村南大街12号中国农业科学院图书馆楼4101-4103室
  • 邮编:100081
  • 邮箱:zgnykx@caas.cn
  • 电话:010-82109808 82106279
  • 国际标准刊号:ISSN:0578-1752
  • 国内统一刊号:ISSN:11-1328/S
  • 邮发代号:2-138
  • 获奖情况:
  • 中国期刊方阵“双高”期刊,第三届中国出版政府奖提名奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),英国食品科技文摘,中国北大核心期刊(2000版)
  • 被引量:85620