中文摘要：

本试验旨在研究中国美利奴羊内皮型一氧化氮合酶（endothelial nitric oxide synthase,eNOS）基因第7、13、14外显子上的单核苷酸多态性（SNPs）和单氨基酸多态性（SAPs）。利用生物信息学方法对人、小鼠和中国美利奴羊eNOS基因上的第7、13、14外显子进行了相似性比较,并对GenBank SNP数据库上已公布的人和小鼠eNOS基因外显子多态性进行了统计分析,然后采用PCR-SSCP方法对120只中国美利奴羊eNOS基因的这3个外显子的扩增片段进行分析,通过分析发现在120只中国美利奴羊这3个外显子上并没有SNPs位点,说明中国美利奴羊eNOS基因上的这3个外显子区域相对保守。

英文摘要：

This experiment was to study the single nucleotide polymorphisms （SNPs） and single amino acid polymorphism （SAPs） of exon 7 ,exon 13 and exon 14 in Chinese Merino sheep endothelial nitric oxide synthase （eNOS） gene, which were an- alyzed according to the published SNPs of exons loci of endothelial nitric oxide gene of human and mouse in GenBank. 3 exons of Chinese Merino sheep eNOS gene, having the higher similarity with the human and mouse, were found through bioinforman- tics method, and they were exon 7,exon 13 and exon 14, respectively. Exons polymorphism in human and mouse eNOS gene were aligned and analyzed by the NCBI SNP database, found that 2 SNPs loci in the human eNOS gene exon 7 （rs1799983） and exonl3 （rs148554851） had clinical significance. After that, the amplified fragments of the 3 exons in Chinese Merino sheep eNOS gene were analyzed by PCR-SSCP method, indicating that SNPs loci were not discovered 3 exons in Chinese Merino sheep, therefore, 3 exons regions of Chinese Merino sheep eNOS gene were relatively conservative.