采用滤纸片法和牛津杯法从多年生人参根际土壤中筛选出1株对立枯丝核菌(Rhizoctonia solani Kuhn)和人参链格孢菌(Alternaria panax Whetz)均有较强拮抗能力的内生芽孢杆菌(Bacillus endophyticus)SZ-56,并测定了其防病促生效果。结果表明:SZ-56对立枯丝核菌和人参链格孢的抑菌率分别为87.77%和65.40%,其还具备广谱拮抗性;SZ-56具备盆栽防控能力,对人参立枯病、人参黑斑病的保护和治疗作用效果分别为70.25%、73.94%和52.9%、53.41%,防治效果与农药对照组相比持平或略高;SZ-56对人参促生效果显著,经其处理后的人参平均植株高、茎叶鲜质量、茎叶干质量、根长、根鲜质量、根干质量均有不同程度的增加,自然土中人参的茎叶鲜质量、根鲜质量和根干质量均超对照2倍以上,其中根干质量增长率更是高达239.99%,显著优于对照组。
By filter paper method and Oxford cup method,a strain of Bacillus endophyticus SZ-56,which was strongly antagonistic to Rhizoctonia solani Kuhn and Alternaria panax Whetz, was screened from ginseng rhizosphere soil,and control ability and growth-promoting effect at the root of the ginseng were determined. The results indicated that the inhibitive rate of the bacterial strain SZ-56 to Rhizoctonia solani Kuhn and Alternaria panax Whetz were 87. 77% and 65. 40% respectively.The bacterial strain SZ-56 had good control effect on ginseng fungal pathogens. The strain SZ- 56 had flat or slightly higher efficacy( P〈0. 05) compared with the pesticides treatments,with no dilution in pots trails,the protective and the therapeutic efficacy of the aseptic filtrate of SZ-56 reached 70. 25% and 73. 94% on Rhizoctonia solani Kuhn,52. 9% and 53. 41% on Alternaria panax Whetz,respectively. The study on the growth-promoting effect indicated that after drenching the ginseng root with SZ-56 was significantly higher than ck in pots trails,the average plant height,stem and leaf fresh weight,stem and leaf dry weight,root length,root fresh weight and root dry weight increased to varying degrees. Wherein,the fresh and dry root mass as well as the dry mass of the stem and leaf in the natural soil was more than 2 times of that of the control plant,the dry mass of the root increased at a rate as high as 239. 99%,and the observation group was significantly better than the control group.