中文摘要：

为了研究牛樟芝（Antrodia camphorata）倍半萜的生物合成,通过对牛樟芝基因组分析获得倍半萜类合成酶基因（AcTPS2）,利用RT-PCR克隆获得其全长cDNA,对其进行生物信息学分析和表达谱分析。结果显示：AcTPS2基因cDNA全长1 068bp,Blast比对发现,AcTPS2含倍半萜类合成酶所独具的富含天冬氨酸序列DXXXD及萜类合成酶特有的RRDTSG-LDL保守序列;系统进化分析显示,AcTPS2基因与其他真菌的倍半萜聚为一类;表达谱分析显示,以甘露糖作为碳源、以酪蛋白胨作为氮源能够有效促进AcTPS2基因的诱导表达。研究结果可为以后牛樟芝倍半萜类生物合成提供一定的参考依据。

英文摘要：

In order to study the biosynthesis of sesquiterpene,this text analyzed the sesquiterpene synthetases gene（AcTPS2）through genome analysis of Antrodia camphorata,and obtained the full length cDNA with RT-PCR,carried on bioinformatics and mRNA analysis.The results showed that AcTPS2 cDNA has1 086bp;Blast analysis revealed that AcTPS2 included abundant asparagic acid motif DXXXD that is specific motif of sesquiterpene synthetase,and terpene synthetase special conserved sequence RRDTSG-LDL;phylogenetic trees showed AcTPS2 and other fungus sesquiterpene synthetase amino acid sequence clustered in one clade;mRNA analysis showed manitol as carbon source,casein peptone as nitrogen source could promote AcTPS2 effectively,and the results could supply some basis in sesquiterpene biosynthesis.