中文摘要：

前期研究从杨柳田头菇（Agrocybe salicacola）YAASM0711的单核菌株M20中获得了含有GC-AG内含子的3-磷酸甘油醛脱氢酶（Glycerol-3-phosphate dehydrogenase,GPD）gpd完整编码基因,本研究通过特异性引物PCR扩增探讨该基因的组成结构及内含子的拼接特征.在杨柳田头菇和茶树菇（A.aegerita）野生群体中均获得了此基因,序列差异性分析显示该基因可被分为两类,并且在两个物种中均有分布,表明这种序列组成差异可能在两个物种分化之前就已经存在.另外,在M20菌株的菌丝体及YAASM0711菌株的不同发育时期均没有发现可选择性剪切现象.基因表达分析表明,gpd在幼嫩的子实体中表达量最高,约为菌丝体中表达量的5.5倍,推测与子实体的快速生长有关.本研究结果有助于提高对gpd功能及其在物种分化研究中作用的认识.

英文摘要：

The rare introns with GC-AG border are found in some organisms including human,Caenorhabditis elegans and filamentous fungi with low detection rate of 0.6%-1.2%.In general,this type of introns seem to be related to the alternative splice,and their retention or removal regulate the function of the translated protein.It is necessary to obtain the glycerol-3-phosphate dehydrogenase（GPD） encoding gene from Agrocybe salicacola strain M20 and to understand its splicing characterization.This study cloned the complete gpd gene containing GC-AG intron from single spore isolation M20 of Agrocybe salicacola YAASM0711 by using PCR with genomic sequencing data.The population investigation showed two types of GC-AG introns amplified by special primers,both found in each of the two species A.salicacola and A.aegerita,which indicated that they probably occurred before the species divergence.In addition,alternative splice was not detected in the mycelia of M20 or any development stage of YAASM0711.Gene expression showed the highest mRNA level of gpd existing in young fruiting body,and probably playing an important role during the rapid growth of fruiting body.To the best of our knowledge,this was the first report on the GC-AG introns in gpd gene.The splicing characterization of glycerol-3-phosphate dehydrogenase GC-AG introns in genus Agrocybe will provide help for further understanding of GC-AG intron in function and evolution.