中文摘要：

目的探讨AngⅡ2型受体（AT2R）基因局部电穿孔转染对大鼠颈总动脉球囊损伤后新生内膜增生的影响。方法建立大鼠颈总动脉球囊损伤模型,用局部电穿孔方法转染AT2R真核表达载体（pEGFP-AT2R）或空载体（pEGFP-N2）,分别于术后7、14 d和21 d采用HE染色及RT-PCR方法进行AT2R、AngⅡ1型受体（AT1R）在颈动脉壁中表达的变化和组织形态学分析,检测其对在体血管新生内膜的影响作用。结果球囊损伤21 d后,pEGFP-AT2R组大鼠颈动脉AT2R mRNA表达为（1.262±0.317）,pEGFP-N2组为（0.396±0.100）,单纯损伤组为（0.410±0.053）,pEGFP-AT2R组与pEGFP-N2组和单纯损伤组相比差异有统计学意义（P〈0.01）;球囊损伤后21 d时AT1R的表达,pEGFP-AT2R组为（0.469±0.065）、pEGFP-N2组为（0.363±0.046）、单纯损伤组为（0.373±0.045）,pEGFP-AT2R组较pEGFP-N2组和单纯损伤组相比差异有统计学意义（P〈0.05）,pEGFP-N2组和单纯损伤组间无统计学差异（P〉0.05）;球囊损伤后21 d,pEGFP-AT2R组的内膜面积与中膜面积比（I/M）为（0.828±0.101）,pEGFP-N2组为（1.432±0.086）,单纯损伤组为（1.515±0.078）,pEGFP-AT2R组较pEGFP-N2组和单纯损伤组相比差异有统计学意义（P〈0.01）。pEGFP-AT2R组与单纯损伤组相比,使实验动物新生内膜增生平均受抑制率达45.35%。结论在体血管局部电穿孔转染AT2R基因可使AT2R在损伤血管组织表达较单纯损伤组明显增加。AT2R基因与AT1R基因之间在表达量上不存在此消彼长的关系。

英文摘要：

Objective To study the effect of electroporation-mediated in vivo local transfection of angiotensin Ⅱ type 2 receptor（AT2R） gene on neointimal hyperplasia in rat common carotid arteries after balloon angioplasty.Methods Seventy-two healthy SD rats were randomly and evenly divided into a control group,an injured group,an empty plasmid group（pEGFP-N2 group）,and an AT2R transfection group（pEGFP-AT2R group）.Balloon catheter denudation of the endothelia of rat common carotid arteries was routinely used to construct a restenosis model.The transfection of plasmids pEGFP-AT2R and pEGFP-N2 was carried out by local electroporation.The injured arteries were collected 7 d,14 d,and 21 d after transfection.AT2R and angiotensin Ⅱ type 1 receptor（AT1R） expression levels in the carotid artery walls as well as histomorphological characteristics were analyzed with RT-PCR and HE staining.The effect of AT2R on neointimal hyperplasia of in vivo arteries was evaluated.Results The AT2R mRNA expression level in the injured rat common carotid arteries of the pEGFP-AT2R group was（1.262±0.317）,indicating remarkable difference from those of the pEGFP-N2 group（0.396±0.100） and injured group（0.410±0.053） 21 d after transfection（P0.01）.The AT1R mRNA expression level in the pEGFP-AT2R group was 0.469±0.065,statistically higher than those of the pEGFP-N2 group（0.363±0.046） and injured group（0.373±0.045） 21 d after transfection（P0.05）.The intima-to-media（I/M） area ratio of the pEGFP-AT2R group（0.828±0.101） showed obvious difference from those of the pEGFP-N2 group（1.432±0.086） and injured group（1.515±0.078） 21 d after transfection（P0.01）.Compared with the injured group,AT2R transfection in the pEGFP-AT2R group inhibited neointimal hyperplasia in experimental animals at a rate as high as 45.35%.Conclusion Electroporation-mediated in vivo AT2R gene transfection can significantly up-regulate AT2R expression in injured common carotid artery tissues of rats.There is no negativ