中文摘要：

目的建立RP-HPLC法同时测定强力天麻杜仲胶囊（天麻、杜仲、当归等）中6种成分的含有量。方法分析采用依利特C18色谱柱（4.6 mm×250 mm,5μm）;流动相乙腈（A）-0.03%磷酸（B）,梯度洗脱;体积流量1.0 m L/min;检测波长220 nm;柱温25℃。结果天麻素、对羟基苯甲醇、松脂醇二葡萄糖苷、阿魏酸、咖啡酸和绿原酸分别在1.010 0~10.100 0μg（r=0.999 6）、0.012 5~0.125 0μg（r=0.999 8）、0.009 8~0.098 0μg（r=0.999 1）、0.032 0~0.320 0μg（r=0.999 1）、0.049 5~0.495 0μg（r=0.999 2）、0.031 6~0.316 0μg（r=0.999 9）范围内呈良好的线性关系,平均回收率分别为97.9%、97.5%、96.7%、97.8%、96.8%、98.2%,RSD分别为0.25%、1.25%、2.19%、0.59%、1.02%、0.90%。结论该方法准确可靠,重复性好,可用于强力天麻杜仲胶囊的质量控制。

英文摘要：

AIM To establish a RP-HPLC method for simultaneously determining the contents of six constitu- ents in Qiangli Tianma Duzhong Capsules （Gastrodiae Rhizoma, Eucommiae Cortex, Angelicae Sinensis Radix, etc. ）. METHODS The analysis was carried out on a 25 ℃ thermostatic Hypersil Cls column （4.6 mm × 250 mm, 5 μm）, with the mobile phase comprising of acetonitrile （A） -0. 03% phosphoric acid （B） flowing at 1.0 mL/min for gradient elution. And the detection wavelength was set at 220 nm. RESULTS Gastrodin, p- hydroxybenzyl alcohol, pinoresinol diglucoside, ferulic acid, caffeic acid and chlogrogenic acid showed good linear relationships within the ranges of 1. 010 0 - 10. 100 0 Izg （r =0. 999 6）, 0. 012 5 -0. 125 0 μg （r =0. 999 8）, 0.009 8-0.098 0 μg （r=0.999 1）, 0.032 0-0.320 0 μg （r=0.999 1）, 0.049 5 -0.495 0 μg （r= 0. 999 2） and 0. 031 6 -0. 316 0 μg （r =0. 999 9） , whose average recoveries were 97.9% , 97.5% , 96. 7% , 97.8% , 96. 8% and 98.2% with the RSDs of 0.25% , 1.25% , 2. 19% , 0. 59% , 1.02% , 0. 90% , respective- ly. CONCLUSION This method is accurate with good reproducibility, which can be used for the quality control of Qiangli Tianma Duzhong Capsules.