中文摘要：

目的：对甘地胶囊的化学成分进行快速分离与鉴别。方法：采用高效液相色谱．电喷雾．高分辨飞行时间质谱（HPLCESI—TOF／MS）联用技术。色谱柱为Agilent Poroshell 120 SB-C18（100mm×3mm，2．7μm），流动相为0．1％甲酸水溶液．乙腈（梯度洗脱），流速为0．4ml／min，检测波长为254nm，柱温为25℃，进样量为10μl；使用ESI离子源，在负离子模式下采集数据，N2流速为10L／min，载气温度为350℃，毛细管电压为4000V，轰击电压为165V，扫描质量范围（m／z）为100～1100amu。结果：推断出甘地胶囊内容物中主要成分有18种，分别为8-表马钱子苷酸、莫诺苷、马钱素、当药苷、芦丁、异毛蕊花苷、山柰酚-3-O-芸香糖苷、山茱萸新苷、黄芩苷、山柰酚-3-O-葡糖苷、2’-羟基-3＇,4’-二甲氧基异黄烷-7-O-β-d-葡糖苷、汉黄芩苷、黄芪甲苷、黄芩素、汉黄芩素、黄芩新素Ⅱ、异黄芪皂苷Ⅰ、乙酰黄芪皂苷Ⅰ。结论：该方法快速、微量、高效，可为甘地胶囊的药效物质基础和质量控制研究奠定基础。

英文摘要：

OBJECTIVE： To separate and identify the chemical constituents of Gandi capsule. METHODS： HPLC-ESI-TOF/ MS was adopted. The determination was performed on Agilent Poroshell 120 SB-C18（100 mm×3 mm, 2.7 μm） column with mobile phase consisted of 0.1% formic acid-acetonitrile （gradient elution） at the flow rate of 0.4 ml/min. The detection wavelength was set at 254 nm, and column temperature was 25℃. The sample size was 10 μl. ESI and negative ion mode were adopted, and the flow rate and temperature of carrier gas （N2） were 10 L/min and 350℃, respectively. The capillary voltage was 4 000 V, the bombardment voltage was 165 V, and scanning proton range were 100-1 100 amu. RESULTS： A total of 18 chemical constituents were identified in Gandi capsule, including 8-epiloganic acid, morroniside, loganin, sweroside, rutin, isoverbascoside, kaempferol 3-rutinoside, cornuside, baicalin, kaempferol-3-glucoside, 2＇-Hydroxy-3＇, 4＇-dimethoxy-isoflavane-7-O-β-d-glucoside, wogonoside, astragaloside, baicalein, wogonin, skullcapflavon Ⅱ, isoastragaloside Ⅰ and acetytastragaloside Ⅰ. CONCLUSIONS： The method is rapid, trace and highly active, and can lay foundation for pharmacodynamic material basis and quality control of Gandi capsule.