中文摘要：

目的 ：探讨日本血吸虫可溶性虫卵抗原（soluble egg antigen,SEA）致敏小鼠骨髓源性树突状细胞（dendritic cells,DCs）后对其细胞表型及Th17细胞分化的影响。方法 ：收集BALB/c小鼠骨髓细胞,应用粒细胞-巨噬细胞集落刺激因子（granulocyte-macrophage colony stimulating factor,GM-CSF）、白介素（interleukin,IL）-4定向诱导小鼠骨髓细胞向DCs分化;流式细胞仪检测SEA刺激培养后DCs表面分子的表达情况;流式细胞术检测DCs分泌IL-6、IL-23的水平,ELISA法检测DCs分泌转化生长因子-β（transforming growth factor-β,TGF-β）的水平;SEA刺激DCs与CD4＋T细胞共培养后检测培养上清中IL-6、TGF-β、IL-23和IL-17细胞因子水平。结果：诱导培养出可供实验用的高纯度DCs;SEA刺激DCs能表达较高水平的CD80、CD86、CD40、组织相容性复合体（major histocompatibility complex,MHC）-Ⅱ类分子;SEA刺激DCs产生IL-6和TGF-β明显增多;SEA刺激的共培养细胞上清中IL-17水平增高。结论：SEA能够促进DCs成熟,并诱导CD4＋T细胞向Th17细胞分化。

英文摘要：

Objective：To observe the functional changes of routine bone marrow derived dendritic cells （DCs） loaded with the sol- uble egg antigen （SEA）, and its impact on the differentiation of Th17 cell. nethods：Murine bone marrow cells were induced to differ- entiate into DCs by GM-CSF and IL-4. Flow cytometry was used to measure IL-6 and IL-23 and＇ELISA was used to measure TGF-β concentrations in the supernatant of cultured DCs. CD4T ceils and DCs were co-cultured and then the concentrations of relevant cy- tokines in the culture supernatant were detected. Results：Highly purified DCs wire induced and could be used for further experi- ments. DCs were stimulated with SEA to express high levels of CD40, CDS0, CD86 and MHC-II. Meanwhile, DCs stimulated with SEA produced significantly higher concentrations of IL-6 and TGF-β than the blank control group. Co-cultured cells stimulated with SEA yielded significantly higher concentrations of IL-17 than the blank control group. Conclusion：Stimulation by SEA generates mature DCs,and can induce the differentiation of CD4CF cells towards Thl7 cells.