中文摘要：

目的：研究周络通胶囊对糖尿病周围神经病变模型小鼠坐骨神经细胞凋亡的保护作用。方法：40只KK/Upj-Ay小鼠随机分为模型（等容蒸馏水）组与周络通高、中、低剂量（6.85、3.43、1.71 g/kg）组,另取10只C57BL/6小鼠作正常对照（等容蒸馏水）组。灌胃给药,每天1次,连续3个月。检测小鼠坐骨神经传导速度（MNCV）;测定小鼠空腹血糖（FBG）值、血液中糖化血红蛋白（HbA1c）含量;取小鼠坐骨神经,用流式细胞术测定细胞凋亡率;RT-PCR和Western blot法测定坐骨神经B细胞白血病/淋巴瘤相关抗原2（Bcl-2）、Bcl-2相关X蛋白（Bax）、半胱氨酸天冬氨酸蛋白酶3（Caspase-3）、凋亡相关因子（Fas）、Fas受体（FasL）mRNA和蛋白的表达。结果：与模型组比较,周络通高剂量组小鼠FBG、血液中HbA1c含量显著减少（P〈0.01或P〈0.05）;周络通高、中、低剂量组小鼠坐骨神经MNCV显著增加,细胞凋亡率显著降低,坐骨神经Bax、Caspase-3、Fas mRNA表达与Bax蛋白表达显著减弱（P〈0.01或P〈0.05）;周络通高、中剂量组小鼠坐骨神经FasL mRNA表达水平增强（P〈0.01）,Caspase-3、Fas、FasL蛋白表达显著减弱,Bcl-2蛋白表达显著增强（（P〈0.01或P〈0.05））;周络通高剂量组小鼠Bcl-2 mRNA表达显著增强（P〈0.01）。结论：周络通胶囊对糖尿病周围神经有一定的保护作用,其机制与上调Bcl-2、Fas和FasL表达,下调Bax、Caspase-3表达,抑制细胞凋亡率有关。

英文摘要：

OBJECTIVE：To investigate the effect of Zhouluotong（ZLT）capsule on sciatic nerve cells apoptosis in diabetic mice.METHODS：40 KK/Upj-Ay mice were randomly divided into model group（constant volume of distilled water）,ZLT low-dose,middle-dose and high-dose groups（6.85,3.43,1.71 g/kg）.Other 10 C57BL/6 mice were selected as normal control group（constant volume of distilled water）.Mice were given medicines intragastrically once a day for consecutive 3 months.The velocity of sciatic nerve conduction（MNCV）in mice were determined.The levels of FBG and HbA1c in blood were determined in mice.The sciatic nerve of mice was collected,and apoptotic rate was detected by flow cytometry.Real-time PCR and Western blot were used to determine the mRAN and protein expressions of Bcl-2,Bax,Caspase-3,Fas and FasL.RESULTS：Compared with model group,the levels of FBG and HbA1c were decreased significantly in ZLT high-dose group（P0.01 or P0.05）;MNCV of sciatic nerve were increased significantly in ZLT high-dose,medium-dose and low-dose groups while cell apoptosis,mRNA expressions of Bax,Caspase-3 and Fas and protein expression of Bax were decreased significantly（P0.01 or P0.05）.mRNA expression of FasL in sciatic nerve of mice were increased significantly in ZLT high-dose and medium-dose groups（P0.01）.The protein expressions of Caspase-3,Fas and FasL in sciatic nerve of mice were decreased significantly in ZLT high-dose and medium-dose groups,while the protein expression of Bcl-2 were increased significantly（P0.01 or P0.05）.The mRNA expressions of Bcl-2 in sciatic nerve of mice was increased significatly（P0.01） CONCLUSIONS：ZLT capsule has protective effects on diabetic peripheral nerve,which may be associated with the up-regulation of Bcl-2,Fas and FasL expression,down-regulation of Bax and caspase-3 expression and the inhibition of cell apoptosis.