中文摘要：

造骨细胞前 MC3T3-E1 房间在玻尿的修改酸的 chitosan/collagen/nano-hydroxyapatite (HA-CS/Col/nHAP ) 是有教养的合成脚手架并且与 phytoestrogen 对待 -zearalanol (为骨头织物工程改进骨头织物形成的 -ZAL) 。灌注和动态紧张被用于三维(3D ) 有教养的房间，它在骨头组织模仿机械微型环境并且解决集体转移问题。在 vitro 的房间支架构造的形态学然后被检验，成骨的标记被 immunohistochemistry 染色并且西方的弄污估计。结果证明房间以一种不规则的方式扩展了他们的 pseudopodia 并且在 3D-dynamic 文化沿着墙分散了。Osteogenic 显型被增加或由提高的骨胶原坚持说(COLI ) 我铺平，减少的 osteopontin 表示和有的很少在 12 天期间在 osteocalcin 上完成表示在 vitro 文化。响应 -ZAL, ，房间支架构造显示出禁止的细胞的增长，提高了碱的磷酸酶(高山) 活动和到原子因素 kappa B (NF-B ) ligand (RANKL ) 的受体使活跃之物的 osteoprotegerin 的增加的比率。到与 -ZAL 对待的房间支架构造的灌注和动态紧张的申请在骨头织物工程的成骨刺激的研究代表一条有希望的途径。

英文摘要：

Pre-osteoblast MC3T3-E1 cells were cultured in hyaluronic acid-modified chitosan/collagen/nano-hydroxyapatite （HA-CS/Coi/nHAP） composite scaffolds and treated with phytoestrogen α-zearalanol （α-ZAL） to improve bone tissue formation for bone tissue engineering. Perfusion and dynamic strain were applied to three-dimensional （3D） cultured cells, which simulates mechanical microenvironment in bone tissue and solves mass transfer issues. The morphology of cell-scaffold constructs in vitro was then examined and markers of osteogenesis were assessed by immunohistochemistry staining and western blotting. The results showed that cells expanded their pseudopodia in an irregular manner and dispersed along the walls in 3D-dynamic culture. Osteogenic phenotype was increased or maintained by enhanced collagen I （COLI） levels, decreased osteopontin expression and having little effect on osteocalcin expression during the 12 days of in vitro culture. In response to α-ZAL, the cell-scaffold constructs showed inhibited cellular proliferation, enhanced the alkaline phosphatase （ALP） activity and increased ratio of osteoprotegerin to receptor activator of nuclear factor kappa B （NF-KB） ligand （RANKL）. Application of perfusion and dynamic strain to cells-scaffold constructs treated with α-ZAL represents a promising approach in the studies of osteogenesis stimulation of bone tissue engineering.