中文摘要：

在双子叶植物中,SEPALLATA3（SEP3）参与生殖生长的很多方面,包括花分生组织和花器官的形成。但是在单子叶植物中,花器官形成的分子机制还不清楚。为了揭示单子叶植物中SEP3蛋白的结构以及聚合体的形成与生物学功能的关系,以单子叶植物绿竹Bambusa oldhami为材料,构建绿竹SEP3（BoSEP3）蛋白不同结构域原核表达载体,在大肠杆菌中进行重组蛋白诱导表达,获得不同结构域的可溶蛋白,通过镍柱及分子筛分离纯化表明,BoSEP3蛋白K结构域以四聚体形式存在;同时构建BoSEP3蛋白不同结构域的酵母表达载体,通过酵母双杂交系统检验BoSEP3蛋白同源互作表明,K结构域有参与形成多聚体的功能。实验提供了一套表达纯化BoSEP3蛋白以及检验蛋白质互作的有效方案,为开展BoSEP3蛋白功能和结构生物学研究奠定了基础。

英文摘要：

SEPALLATA3 （SEP3） protein plays important roles in many aspects of the reproductive growth in dicotyledonous plants, including formation of flower meristems and organs. However, the molecular mecha- nism of floral organ formation in monocotyledon plants is not very clear yet. To reveal the structural charac- teristics of SEP3 as well as its relationship between the potential polymer formation and biological functions, a series of experiments using the monocotyledon plant Bambusa oldhami as the material were performed. Different protein domains were constructed and soluble expressions were obtained in prokaryotic E. coil sys- tem. The target proteins were purified through one-step nickel-affinity approach. Gelfihration assay indicat- ed that K domain was involved in the tetrameric formation under the testing conditions. Furthermore, the in- volvement of the K domain in the polymer formation was verified by yeast two-hybrid system. Thus an effec- tive approach to express and purify Bantbusa oldhami SEP3 （BoSEP3） protein was established. Confirmation of BoSEP3 as a tetramer in vitro facilitates a functional and structural analysis in the future.