研究经地肤子活性提取物处理后山楂叶螨的症状表现,并采用生化方法测定地肤子活性成分对山楂叶螨体内的蛋白含量、蛋白酶活性、谷胱甘肽-S-转移酶、乙酰胆碱酯酶、单胺氧化酶活性的影响。结果表明:地肤提取物处理山楂叶螨后,螨体内蛋白含量、蛋白酶活性有所升高,谷胱甘肽-S-转移酶活性被激活,这表明地肤提取物对山楂叶螨有毒害作用,从而引起了螨体的解毒作用,来维持正常的生理功能;而乙酰胆碱酯酶、单胺氧化酶的活力受到一定程度的抑制造成神经传递的阻断,从而导致螨体的死亡。地肤子作为新型植物源农药具有良好的开发前景。
The symptom of Tetranychus viennensis treated with KFCE-2 was investigated in this experiment. Several important enzyme systems, protein content, protease, glutathione-S-transferase (GSTs), acetylcholine esterase (AchE) and mnoamine oxidase, in T. viennensis treated with chloroform extract of Kochia scoparia were measured. The results that the protein content and the activity of protease in T. viennersis treated with the extracts of K. scoparia were hoisted and the activity of GSTs was induced indicated that some poisonous components in K. scoparia had aroused the detoxification of T. viennensis. But the activities of AchE and monoamine oxidase in T. viennensis were inhibited. The inhibiting effects of AchE and monoamine oxidase meant the transfer of nerve impulse was interdicted and then caused the death of T. viennensis.