中文摘要：

固定化酶具有高效催化、可重复利用及易分离等优点,在食品、医药等工业生产领域及微观生化检测领域具有广泛应用。固定化酶的性能与载体材料关系密切。石墨烯作为一种广受关注的纳米材料,其独特而优良的物化性能是固定酶的理想载体。本文主要介绍了近年来以石墨烯及其衍生物为载体的固定化酶技术的发展状况。重点对以物理吸附法、化学键合法和包埋法为主的各种石墨烯固定化酶的制作方法,以及其在酶微反应器和生物传感等领域的最新应用进行详细的评述,并对其今后的发展前景进行了展望。

英文摘要：

The immobilized enzyme has been widely applied to the food and medicine industrial production,and micro biochemical detection field for its advantages of high efficiency catalysis,recyclability and easy separation. The performance of immobilized enzyme is closely related to the carrier materials. As a new type of nano materials,graphene has excellent physical and chemical properties,which is an ideal carrier for the immobilization of enzyme.This article focused on the technology of immobilized enzyme using graphene as carrier for the past few years. The immobilization methods including physical adsorption,chemical bonding and encapsulation were particularly discussed. The recent applications of immobilized enzyme in the field of enzyme micro-reaction and biosensor were reviewed. The prospects of its future development were also outlined.