中文摘要：

目的：研究恶性肿瘤患者血栓前状态（prethrombotic state,PTS）分子标志物变化并探讨其意义。方法：采用流式细胞仪、酶联免疫吸附双抗体夹心法（enzyme-linked immunosorbnent assay,ELISA）、放射免疫法、全自动血凝分析仪等检测恶性肿瘤患者血小板表面P-选择素（P-selectin,CD62P）、血浆组织型纤溶酶原激活剂（plasma tissue plasminogen activator,t-PA）、血清内皮素（endothelin,ET-1）及凝血4项[血浆纤维蛋白原（fibrinogen,FIB）水平、活化部分凝血活酶时间（activated partial thromboplastin time,APTT）、凝血酶原时间（prothrombin time,PT）、凝血酶时间（thrombin time,TT）]。结果：肿瘤组CD62P,FIB,ET-1均较正常组增高（P〈0.05）,肿瘤组t-PA水平较正常组降低（P〈0.05）;肿瘤组APTT和PT时间明显缩短（P〈0.05）,TT时间与正常组比较无统计学意义（P〉0.05）。结论：恶性肿瘤患者出现血小板活化、凝血功能增强、纤溶功能减退,存在明显的血栓前状态。

英文摘要：

Objective To study the changes and significance of prethrombotic state indexes in patients with malignant cancer.Methods The expression of CD62p on platelets,level of plasma tissue plasminogen activator（t-PA）,level of serum endothelin（ET-1）,activated partial thromboplastin time（APTT）,fibrinogen（FIB）,prothrombin time（PT）,and thrombin time（TT）were examined by flow cytometer,enzyme-linked immunosorbnent assay,radioimmunoassay method,and auto coagulometer.Results The average expression of CD62P,level of plasma FIB and serum ET-1 in the tumor group increased compared with those in the control group（P〈0.05）;the level of t-PA in the tumor group decreased compared with that in the control group（P〈0.05）.APTT and PT were significantly shortened in the tumor group（P〈0.05）.There was no significant changes of TT in 2 groups（P〉0.05）.Conclusion The platelet is activated,the thrombin activity increases,and the fibrinolytic function declines in patients with malignant cancer;there is an obvious prethrombotic state in patients with malignant cancer.