中文摘要：

目的研究高迁移率族蛋白1（highmobilitygroupbox-1，HMGBl）mRNA及蛋白在喉鳞状细胞癌（简称喉鳞癌）组织及患者血清中的表达及其临床意义。方法反转录聚合酶链反应（RT-PCR）及Westernblot分别检测HMGBlmRNA及蛋白在30例喉鳞癌患者肿瘤组织及癌旁黏膜组织中的表达；酶联免疫吸附试验（ELISA）检测HMGBl蛋白在喉鳞癌患者及正常志愿者血清中的表达。结果RT．PCR结果示HMGBlmRNA（HMGBl／GAPDH）在喉鳞癌组织、癌旁黏膜组织中的相对表达量（x±s）分别为1．25±O．12、0．32±0．04，差异有统计学意义（t=40．27，P〈0．05）；Westernblot结果示HMGBl蛋白（HMGBl／β-actin）在喉鳞癌和癌旁黏膜组织中的相对表达量分别为1．294-0．10、0．34±O．03，两组问差异有统计学意义（t=49．84，P〈0．05）。结果显示HMGBlmRNA和蛋白的表达水平与患者的T分级、临床分期、有无淋巴转移及是否吸烟有关（P值均〈0．05）；而与年龄、性别、是否饮酒及肿瘤病理分级无关（P〉0．05）。ELISA结果显示，喉鳞癌患者及正常志愿者血清中HMGBl质量浓度分别为（24．80±14．08）ng／ml、（23．58±14．69）ng／ml，差异无统计学意义（t=0．37，P〉0．05）。结论HMGBlmRNA及蛋白在喉鳞癌组织中的表达显著高于癌旁黏膜组织，且表达水平与T分级、临床分期及有无淋巴转移有关。

英文摘要：

Objective To investigate the mRNA and protein expressions of high mobility group box- 1 （HMGB1） in the tumor tissues and sera of patients with laryngeal squamous cell carcinoma （LSCC） and their clinical significance. Methods Semi-quantitative reverse transcriptase-polymerase chain reaction （RT-PCR） and Western blot were used to detected the expressions of HMGB1 mRNA and protein in the tumors and adjacent normal epithelial tissues in 30 patients with LSCC. Serum HMGB1 protein levels in the patients with LSCC and in 10 healthy volunteers were detected with enzyme-linked immunosorbent adsorption experiment（ ELISA ）. Results RT-PCR demonstrated that the mean relative mRNA expression levels of HMGB1 （HMGB1/GAPDH） in LSCC tissues and in adjacent normal epithelial tissues were 1.25±0.12 and0.32 ±0. 04, respectively （ t = 40.27, P 〈 0.05 ）. Western blot revealed that the mean relative protein expression levels of HMGB1 （HMGB1/β-actin） were 1.29 ± 0.10 and 0.34±0.03 （ t = 49.84, P 〈 0.05 ） , respectively. Both mRNA and protein expression levels of HMGB1 were associated with T stage, clinical stage, lymph node metastasis status and smoking （ all P 〈 0.05 ）, but no significant correlation with age, alcohol consumption and primary tumor grade and location （ all P 〉 0.05 ）. Mean serum HMGB1 protein levels in patients with LSCC and healthy volunteers were （24.80± 14.08 ） ng/ml and （23.58 ± 14.69 ） ng/ml （t = O. 37, P 〉 0.05 ）. Conclusions Both mRNA and protein expressions of HMGB1 were obviously elevated in LSCC. which were associated closelv with T stage,clinical stage and lymph node metastasis.