中文摘要：

目的研究四川白鹅铁蛋白重链（Ferritinheavychain，FHC）基因编码区序列特征及其组织表达差异。方法应用分子生物学技术克隆FHC编码区序列并分析预测其编码蛋白质的结构和功能，实时荧光定量PCR技术定量检测四川白鹅13种组织中FHC的表达量。结果四川白鹅FHC编码区序列为549bp，编码182个氨基酸，其蛋白质分子质量为21345．9，理论pI值为5．74。四川白鹅FHC氨基酸序列与北京鸭和原鸡的相似性最高（分别为100％和97％），与北京鸭具有最近的亲缘关系。FHC是稳定的可溶性蛋白质，含有高度保守的2个铁蛋白铁离子结合结构域标签和7个亚铁氧化酶双铁离子结合位点。在所检测的四川白鹅13种组织中，FHC基因均有表达，并且不同组织中FHC表达量存在明显差异。四川白鹅脾脏和视网膜中FHC的表达量显著高于其他各组织（P〈0．05），肝脏、垂体和肾脏次之，肌肉中FHC表达量最低。结论四川白鹅FHC是高度保守的、组织特异性表达的基因，具有维持铁稳态和参与抗氧化的双重功能，这为阐明鹅FHC功能和铁代谢机制的研究奠定了理论基础。

英文摘要：

Objective To elucidate the molecular characteristic and expression profiling of ferritin heavy chain （FHC） in various tissues of Sichuan white goose. Methods The coding sequence of FHC was cloned, as well as the structure and function of FHC was predicted by molecular biological techniques. The expression levels of FHC gene in 13 tissues of Si- chuan white goose were determined by quantitative real-time PCR. Results The length of the coding region of Sichuan white goose FHC gene was 549 bp, encoding 182 amino acids with predicted molecular weight 21345.9 and pl 5.74. The a- mino acid sequence of goose FHC had the highest similarity with Beijing duck and Gallus gallus （ 100% and 97%, respec- tively） in GenBank, and the closest relationship of FHC was observed between Sichuan white goose and Beijing duck. FHC was a stable souble protein, which included 2 highly conserved ferritin iron-binding regions signatures and 7 ferroxidase di- iron binding sites. Although FHC was found to be expressed in all the examined tissues, the expression levels were signifi- cantly different. The highest expression of FHC was observed in spleen and retina, followed by liver, pituitary, kidney, and muscles. Conclusion FHC gene of Sichuan white goose is a highly conserved, tissue-specifically gene, which plays a key role in controling the balance of labile iron pool and protecting cells from oxidative stress. This will provide a basis for func- tional research of FHC gene and the metabolic mechanism of iron in geese.