中文摘要：

本研究观察四合木在MS＋2，4-D0．5mg／L＋6BA0．1mg／L的悬浮培养下愈伤组织的无丝分裂和对MS＋2，4-D0．1mg／L＋6BA0．25mg／L＋CH500mg·L^-1培养基悬浮培养下体细胞胚胎发生，表明：四合木悬浮培养下细胞经无丝分裂可导致脱分化，细胞分裂时产生不同的细胞特征。亚显微结构观察表明体细胞胚胎发生过程的细胞器和细胞内含物的变化，有利于代谢产物的积累。

英文摘要：

Tetraena mongolica Maxim. was cultured in liquid suspension culture medium composed of MS ＋ 24D0.5mg/L ＋ 6BA0. 1mg/L. Amitosis process of the callus was observed under invertor - microscope. The somatic embryo culture in the medium of MS ＋ 2,4-D0. 1mg/L ＋ 6BA0.25mg/L ＋ CH500mg·L^-1 was observed with electron microscope（TEM）. The result showed that amitosis could lead up to cell dedifferentiation that made the cells display different characteristics. The changes of cell organs and internal materials during development of somatic embryo observed with TEM indicated that the process was beneficial to accumulation of metabolic products.