中文摘要：

目的：探讨不同的免疫途径对抗原肽修饰树突状细胞（DC）疫苗免疫效果的影响。方法：用鸡卵清蛋白（OVA）MHCI限制性多肽SIINFEKL（OVAp257-264）包被小鼠骨髓来源的DC，通过皮下、腹腔、静脉或肌肉注射免疫小鼠，7天后行体内细胞毒性T淋巴细胞杀伤实验（InvivoCTL）分析CTL杀伤活性和细胞内IFN-γ染色（ICS）分析免疫小鼠脾脏CD8^＋细胞产生IFN-γ的情况。结果：免疫7天后，InvlvoCTL结果显示SIINFEKL修饰的DC皮下、腹腔、静脉或肌肉注射免疫小鼠其特异性CTL杀伤效应分别是37．3％±7．3％、61．0％±4．2％、56．9％±3．6％和10．8％±2．3％；ICS结果示四组小鼠产生IFN-γ的CD8^＋细胞占总CD8^＋细胞的比例分别是0．31％±0．07％、0．85％±0．12％、0．76％±0．14％和0．15％±0．04％。结论：不同免疫途径可明显影响抗原肽修饰DC疫苗的免疫效应，其中腹腔注射诱发的抗原特异性CTL反应最强，静脉注射者次之，而皮下注射特别是肌肉注射较弱，提示通过腹腔注射DC疫苗可能是安全、高效的免疫途径。

英文摘要：

Objective：To investigate the influence of different routes of administration on vaccine efficiency of peptide-pulsed dendritic cells（DC）. Methods： Immunization of syngeneic C57BL/6 mice with bone marrow-derived murine DC pulsed with SIINFEKL, a MHC class I -restricted peptide from chicken ovalbumin（OVA） sited at 257-264 amino acid residuals were performed via subcutaneous（ s. c. ） , intramuscular（ i. m. ） , intravenous（ i. v. ） , and intraperitoneal（ i. p. ） administration, respectively. Seven days later the mice were sacrificed and the splenocytes were prepared to analyze antigen-specific CTL lysis for target cells and IFN-γ-produeting CD8^＋T lymphocytes using in vivo CTL assay and intraceUular cytokine staining（ICS） , respectively. Results：The restdts from in vivo CTL assay showed that the CTL lysis activities were 37.3% ±7. 3%, 10. 8% ±2. 3%, 56.9% ±3.6% and 61.0% ±4. 2% via s. c. , i.m. , i.v. , and i.p. administration, respectively. Similarly, ICS showed that IFN-γ-producting cells in total CD8 ^＋T lymphocytes were 0. 43% ± 0. 09%, 0. 85% ＋ 0. 12%, 0. 76% ＋ 0. 14% and 0. 15% ±0. 04%, respectively. Conclusion： Different routes of administration has an obvious influence on vaccine efficiency of peptide-pulsed DC. The i. p. immunization with DC elicites the strongest CTL lysis activity, i.v. immunization is next, and s. c. , in particular i.m. is the worst, suggesting that i.p. administration may be a safe and effective route for immunization with peptide-pulsed DC against, cancers in a mice model.