中文摘要：

一个满足的绿内障模型是的背景这研究的不在的 now.The 目的是在老鼠评估硅酮油的 intracameral 注射和角膜的 limbal 容器和 episcleral 静脉的电凝法的联合的效果证实绿内障 model.Methods 手术在每 90 成年男 rats.Right 的左眼睛被动当 intraocular 压力( IOP )的 controls.Measurement 与一个 applanation 音调测定器(Tong钢笔)被执行，眼睛被使用 .Retinal 中心房间( RGC )我们

英文摘要：

Background A satisfied glaucoma model is absent now.The aim of this study was to evaluate the effect of a combination of intracameral injection of silicone oil and electrocoagulation of corneal limbal vessels and episcleral veins in the rats to establish glaucoma model.Methods Operation was performed in each of the left eyes of 90 adult male rats.Right eyes were used as controls.Measurement of intraocular pressure （IOP） was performed with an applanation tonometer （Tong-Pen）.Retinal ganglion cells （RGCs） were retrogradely labeled by applying FluoroGold onto the bilateral superior colliculus.Results During the follow-up （24 weeks）,the lOP of the study eyes was significantly higher （P〈0.05） than the control eyes （at final examination,IOP of control eyes was （13.4±1.0） mmHg and lOP of study eyes was （16.1±1.8） mmHg）.Correspondingly,at 24 weeks after operation,the RGCs density of the study eyes （2286.11±290.45/mm^2） was significantly lower than the control eyes （2626.46±164.85/mm^2,P〈0.01）.In the operated eyes,histological examination showed excavation of optic disc and increased neuroglial cells in the optic nerve,reduced thickness of retina and diminution of retinal ganglion cells,and atrophy of ciliary body and iris.Notably,the anterior chamber angle of the operated eye remained open.Conclusions A combination of intracameral injection of silicone oil and electrocoagulation of comeal limbal vessels and episcleral veins may establish a reliable glaucoma model for further research.