中文摘要：

瞄准：在 Huh7 房间在 Bcl-2 表示的 Huh7 房间，和变化上调查 Gli-1 小干扰 RNA (siRNA ) 的效果。方法：人的肝细胞癌房间 Huh7 被使用。房间生存能力被 3- 分析(4， 5-Dimethylthiazol-2-yl )-2, 5-diphenyl tetrazolium 溴化物(MTT ) 试金。Gli-1 和 Bcl-2 家庭成员的表情被 RT-PCR 和西方的污点检测。Apoptosis 被流动血细胞计数用 propidium 碘化物检测，由染色的 Hoechst 33258 测量了使用先进萤光显微镜检查和 caspase-3 酶的试金。细胞生长是有 Gli-1 siRNA 和 5 氟尿嘧啶(5-Fu ) 的分析术后疗法。结果：在通过 Gli-1 siRNA 的 Huh7 房间的 Gli-1 mRNA 的抑制减少了房间生存能力。Gli-1 siRNA 处理也由三个标准， sub-G1 房间周期部分的增加，原子冷凝作用，典型地代表 apoptosis 的一个词法变化，和 caspase-3 的激活导致了 apoptosis。Gli-1 siRNA 对下面调整 Bcl-2 也有能力。然而， Gli-1 siRNA 在 Bcl-xl 导致了没有重要变化， Bax，坏，并且出价。而且， Gli-1 siRNA 在 Huh7 房间上增加了 5-Fu 的细胞毒素的效果。结论：Bcl-2 的下面规定在 Gli-1 siRNA 在 HCC 房间导致的 apoptosis 起一个重要作用。有化学疗法的药的联合 Gli-1 siRNA 能对 HCC 代表更有希望的策略。策略的效果需要推进调查在活体内并且可以有潜在的临床的申请。

英文摘要：

AIM： To investigate the effects of Gli-1 small interference RNA （siRNA） on Huh7 cells, and the change of Bcl-2 expression in Huh7 cells. METHODS： Human hepatocellular carcinoma cells Huh7 were used. Cell viability was analyzed by 3-（4, 5-Dimethylthiazol-2-yl）-2, 5-diphenyl tetrazolium bromide （MTT） assay. The expressions of Gli-1 and Bcl-2 family members were detected by RT-PCR and Western blot. Apoptosis was detected by Flow cytometry using propidium iodide, measured by Hoechst 33258 staining using Advanced Fluorescence Microscopy and caspase-3 enzymatic assay. Cell growth was analyzed after treatment with Gli-1 siRNA and 5-fluorouracil （5-Fu）. RESULTS： Inhibition of Gli-1 mRNA in Huh7 cells through Gli-1 siRNA reduced cell viability. Gli-1 siRNA treatment also induced apoptosis by three criteria, increase in the sub-G1 cell cycle fraction, nuclear condensation, a morphologic change typical of apoptosis, and activation of caspase-3. Gli-1 siRNA was also able to down-regulate Bcl-2. However, Gli-1 siRNA resulted in no significant changes in Bcl-xl, Bax, Bad, and Bid. Furthermore, Gli-1 siRNA increased the cytotoxic effect of 5-Fu on Huh7 cell. CONCLUSION： Down-regulation of Bcl-2 plays an important role in apoptosis induced by Gli-1 siRNA in HCC cells. Combination Gli-1 siRNA with chemotherapeutic drug could represent a more promising strategy against HCC. The effects of the strategies need further investigation in vivo and may have potential clinical application.