中文摘要：

为了解龙眼生长素受体基因TIR1的功能,以龙眼胚性愈伤组织为材料,在转录水平上对TIR1（命名为Dl TIR1-3）及其启动子进行克隆和分析,并在转录后水平上研究TIR1与mi R393的相互调控关系.结果表明,Dl TIR1-3 c DNA全长2196 bp,包含ORF 1 716 bp,编码571个氨基酸（Gen Bank登录号：KR558761）;生物信息学分析发现,Dl TIR1-3属于不稳定蛋白,不含有信号肽,含有跨膜螺旋结构,具有F-box保守结构域和富含异亮氨酸重复结构,且Dl TIR1-3与碧桃TIR1保持较高的同源性.染色体步移法克隆获得Dl TIR1-3启动子序列2909 bp（Gen Bank登录号：KR558763）,该启动子不存在Cp G岛但含有大量光反应、激素应答、组织特异性调控、胁迫响应以及其他生长发育相关的作用元件.ps RNAtarget结合改良RLM-RACE验证表明mi R393能够裂解Dl TIR1-3从而抑制其m RNA转录;q PCR结果显示,Dl TIR1-3与mi R393相互平衡能够调节龙眼体胚形态建成及响应激素调控.由此可见,Dl TIR1-3通过转录水平的调控作用在龙眼生长发育、激素应答、组织分化及胁迫响应等过程中发挥重要功能,而在转录后水平上mi R393通过裂解Dl TIR1-3参与相关调控过程.

英文摘要：

This study aimed to investigate the functions of TIRl in longan.The TIRl（named DlTIRl-3） and its promoter were cloned and analyzed at the transcriptional level in embryogenic callus of Dimocarpus longan Lour.,then the relationship between TIRl and miR393 was researched at the post-transcriptional level.The results showed the cDNA sequence length of DlTIRl-3 as 2196 bp,containing ORF sequence 1716 bp,encoding 571 amino acids（GenBank accession number KR558761）.Bioinformatics analysis showed that DlTIRl-3 was unstable protein,with transmembrane structure and signal peptide,F-box conserved domain and leucine-rich repeats.In addition,DlTIRl-3 shared higher homology with TIRl of Prunus persica.By using the Genome Walking method,2909 bp of DlTIRl-3 promoter was amplified（GenBank accession number KR558763）.Without an CpG island,DlTIRl-3 had multiple cis-elements correlated to plant growth and development,including light response,hormone response,tissue specific regulatory and stress response.According to the result of psRNAtarget and RLMRACE,DlTIRl-3 was regulated by miR393 in longan.The qPCR results revealed that DlTIRl-3 and miR393 kept a mutual equilibrium to regulate the morphological formation of somatic embryogenesis and responses to hormone in longan.Therefore,DlTIRl-3 plays an important role in the growth,hormone response,tissue differentiation and stress response of longan at the transcriptional level,and miR393 may crack DlTIRl-3 to involve in the regulation at the post-transcriptional level.