以黄萎病高抗品种‘新陆早33’和易感品种‘新陆中36’为对照,采用无底营养钵浇根和田间自然病圃相结合的鉴定方法,用发病率、病情指数、抗指、抗效4个鉴定指标为依据,在新疆植棉模式下对来源于同一背景的8个陆地棉染色体片段置换系的抗黄萎病性进行了初步检测和鉴定。结果表明,抗指所鉴定的反应型与病情指数鉴定的反应型一致,抗效的鉴定反应型的标准要低于抗指的鉴定反应型标准,花铃期的鉴定标准要低于苗期的鉴定标准;代号为CSSL155的置换系的反应型为抗病类型,可以作为抗病品种资源进行利用;对苗期的鉴定指标和花铃期的鉴定指标进行差异显著性分析,发现可以用苗期的鉴定预测成株期的发病情况,为常规抗病育种提供理论依据。
Eight chromosome segment substitution lines (CSSL) of Gossypium hirsutum source of the same background were tested for resistance to Verticillium wilt,which contrasted with high resistance to Verticillium wilt ' Xin Lu-zao 33 ' and susceptibility to Verticillium wilt ' Xin Lu-zhong 36 ' in Xinjiang province.Sprinkle the strain suspention on root in non-bottom plastic film bottle and natural disease garden methods were selected to test the resistance.The rate of disease,disease index,relative resistant index and countermeasure effectiveness were adopted in the original text.The discovery showed that infection type of relative resistance index was consistent with disease index.The infection type of countermeasure effectiveness was lower than the infection type of relative resistance index.The qualification standard of flower and boll stage was lower than that of seedling.The results demonstrated that CSSL of code No.CSSL155 could be selected as resistant variety to Verticillium wilt;correlativity analysis on the appraisal that seedling stage appraisal index and flower bell expected that we could use the seedling stage appraisal to forecast fully grown plant scheduled time,the disease-resistant breeding provides the theory basis to routine;at the same time this testing provided the theoretical basis of conventional breeding for Verticillium wilt disease resistance.