中文摘要：

目的：探讨电针对局灶性脑缺血大鼠海马微循环和葡萄糖代谢的改善作用。方法：采用线栓法制备局灶性脑缺血模型大鼠，随机分为假手术对照组、模型组、内关组和地机组，电针治疗15天和30天，应用激光多普勒微循环血流仪检测电针对局灶性脑缺血大鼠海马微循环血流量的影响，采用酶化学法测定脑组织葡萄糖、乳酸及丙酮酸的含量研究电针对脑组织葡萄糖代谢的改善作用。结果：针刺治疗后，针刺内关组大鼠海马CA1区微循环血流量显著高于模型组和针刺地机组大鼠（P〈0．05），针刺内关组大鼠脑组织内葡萄糖和丙酮酸的含量明显升高，乳酸含量降低，与模型组、针刺地机组有显著性差异（P〈0．05），且30天组疗效好于15天组。结论：电针可明显提高MCAO大鼠海马CA1区的微循环血流量，通过微循环的改善进而增加缺血脑组织能量供应，改善缺血后组织细胞的葡萄糖代谢，减轻组织细胞的损伤，促进神经功能的恢复。

英文摘要：

Objective：To study electroaeupuneture on the function mechanism of microcireulatory blood flow in hippocampal CAI region of rats with focal cerebral isehemia caused by middle cerebral artery occlusion （MCAO）,and to study the function mechanism of cerebral glucose metabolism after electroacupuneture. Methods ： Focal cerebral ischemia was induced by the middle cerebral artery occlusion model in Wistar rats. These rats were randomly divided into eight groups ： sham operation groups, model groups, Neiguan groups and Diji groups. Electroaeupuncture was applied for fifteen days and thirty days after MCAO. The change of mieroeireulatory blood flow in hippocampal CA1 area was monitored by laser-Doppler Flow meter（LDF） on the fifteenth and thirtieth days after MCAO respectively in each group. The content of Glucose, Pyruvic Acid and Lactic Acid in brain tissue was analyzed with specialized measuring kits. Results： Microcircnlatory blood flow in hippocampal CA1 region of MCAO rats was obvi- ously increased after electroacupuncture at Neiguan acupoint than that was in the model group, this effects were more obveriously than the effects in Diji group（ P 〈 0.05）. Compared with the model group and the Diji group,The content of Glucose and Pyruvie Acid were increased in Neiguan groups （ P 〈 0.05）. The content of Lactic Acid in brain tissue were decreased after acupuncturing at Neiguan（ P 〈 0. 05） . Conclusion：Microcireulatory blood flow in hippocampal CA1 region of MCAO rats could be increased by acupuncture. Through the improvement of microcirculation leading to the increased energy supply of ischemic brain,then the improvement of glucose metabolism in ischemic brain tissue could reduce neuronal injury and promote recovery of neurological function.