中文摘要：

目的：研究单纯缺血负荷对家兔股动脉固有侧支循环开放程度和开放持续时间的影响，为生理性缺血训练的深入研究提供依据。方法：健康成年家兔26只，体重（2．5±0．5）kg。股动脉以动脉夹夹闭，制作下肢单纯缺血模型，逐级设定不同缺血时间、间隔和缺血频率。采用微球技术检测缺血区和正常区股动脉固有侧支循环血流（FCBF）。结果：①FCBF在阻断后递增，第3min时达到高峰，较阻断后即刻明显增加（P〈0．01），第4min时达到平台期。②缺血区在阻断3min后再灌注，再灌注第2min时局部血流较阻断前明显增加（P〈0．01），再灌注最大维持时间可以达到5min，不超过8min。⑧反复缺血刺激（阻断3min，间隔5min）6次，缺血3次时FCBF显著增加，达到高峰（P〈O．01），继续增加缺血频率FCBF反而呈下降趋势。结论：家兔股动脉最大FCBF开放的最短缺血时间为3min，再灌注最大维持时间可以至5min，适宜缺血频率为3次。

英文摘要：

Objective： To study the minimal ischemic burden for evoking maximal opening of intrinsic femoral collaterals and provide the basis for clinical application of physiological ischemic training. Method： Twenty-six New Zealand adult rabbits [（2.5±0.5）kg] were subjected to complete occlusion of femoral artery with different isehemic burden （graded ischemie duration, interval and frequency）. Femoral artery collateral blood flow （FCBF） was measured with microsphere technique. Result： 1. FCBF increased progressively at the onset of occlusion and reached the peak level at the 3rd min （P〈 0.01）, then entered the plateau phase with occlusion prolonging at the 4th min. 2. Compared to pre-oeclusion, the regional blood flow increased significantly at the 2nd rain after one episode of 3min ischemia （P〈0.01）. The maximal opening of intrinsic femoral collaterals maintained at least 5 min, but decreased gradually at the 8th min after reperfusion. 3. FCBF increased significantly after three repetitive 3min occlusions with 5min interval between each （P〈0.01）. A trend of decrease was shown with the isehemic burden reaching more than 3 times. Conclusion： The data suggests that the fully opening of intrinsic collaterals circulation after artery occlusion requires 3min duration and maintains at least 5 min, which is augmented by three repetitive 3min occlusions with 5min interval. Increase of ischemie burden can not enhance FCBF further.