中文摘要：

目的：应用梯度洗脱高效液相色谱法，对2种制备工艺的银黄方中有效成分及含量进行分析比较。方法：采用DiamonsilC18（4．6mm×250mm，5μm）色谱柱，乙腈-0．1％磷酸溶液作流动相梯度洗脱，以流速1．0mL·min6-1，在检测波长276nm和328n／n处测定银黄方中有效成分绿原酸、黄芩苷和汉黄芩苷的含量。结果：绿原酸在0．0118～0．059μg线性关系良好（r=0．9999），平均回收率为98．30％；黄芩苷在0．19～9．5μg线性关系良好（R=0．9999），平均回收率为102．87％；汉黄芩苷在0．0104～0．52μg线性关系良好（r=0．9999），平均回收率为104.33％。银黄方膜分离工艺得到3种成分固含量总和（〉50％）高于银黄口服液（13．36％）。结论：本法可用于银黄方中上述3种成分的含量测定，膜分离工艺得到的有效部位含量占提取物的50％以上。

英文摘要：

Objective：To establish an HPLC method by gradient elution for comparative analysis of the ef- fective composition in two kinds of Yinhuang decoction. Method： The column was Diamonsil C18 （4. 6 mm × 250 Mm,5 μm）. The mobile phase was consisted of acetonitrile-0.1% phosphoric acid with gradient elution. The flow rate was 1.0 mL.min-1 and the detection wavelength were at 276 nm and 328 nm for the content determination of chlorogenic acid, baicalin and wogonoside. Results： Chlorogenic acid, baicalin and wogonoside were linear in the range of 0. 011 8-0. 059 μg（r = 0. 999 9） ,0. 19-9.5 μg（r = 0. 999 9） and 0. 0104-0.52 μg（r = 0. 999 9 ） respectively. The average recoveries of them were 98.30% , 102.87% and 104.33% respectively. The total solid content of Yinhuang decoction separated by filtration membrane was more than 50% compared with that of Yinhuang oral liquid（13.36% ）. Conclusion： The HPLC can be applied to content determination of chlorogenic acid, baicalin and wogonoside in Yinhuang decoction, the content of effective parts separated by filtration membrane was more than 50%. Filtration membrane was conducive to developing the fifth class new drugs.