中文摘要：

研究了曙红Y（EOSY）与牛血清白蛋白（BSA）作用的荧光光谱和紫外可见吸收光谱特征，实验证明EOSY具有较强地猝灭BSA荧光强度的能力，分别用Stern-Volmer方程、Lineweaver-Burk方程和热力学方程等处理试验数据，得到了25℃时反应的结合常数为3．601×10^5L·mol^-1，反应的△H^θ，△G^θ和△S^θ分别为-20．66，-31．70kJ·mol^-1和37．06J·K^-1，为研究在人体生理条件下EOSY与BSA的结合方式、EOSY对BSA构象影响和细胞染色机理等提供了重要信息。

英文摘要：

The binding of eosin Y to bovine serum albumin （BSA） was studied by fluorescence spectroscopy and ultraviolet spectrum. It was shown that this compound has a quite strong ability to quench the fluorescence from BSA. After analyzing the fluorescence quenching data according to Sterm-Volmer equation, Lineweaver-Burk equation and thermodynamic equation at 298. 15 K, the binding constant （KLB = 3. 601 ×10^5L · mol^-1 ） and thermodynamic parameters （△H^θ, ： - 22.66 kJ ~ mol 1, △G^θ： -31.30 kJ · mol 1 and △S^θ： 36. 32 J · K^-1）were obtained, which provide important information for researching the configuration modification of BSA caused by added eosin Y, biological effects of eosin Y in a living body, and dyeing mechanism of cells.