在开发玉米核的充满的率和淀粉累积被分析。与蔗糖新陈代谢和淀粉生合成联系的酶活动的变化被调查。目的为淀粉合成讨论酶的机制负责。玉米栽培变种(Zea mays ) 的二种类型,高淀粉玉米(Feiyu 3 ) 和正常玉米(Yuyu 22 ) ,在一块玉米地被种。涉及淀粉合成的因素在生长时期期间被执行。充满率,蔗糖内容,积累率的淀粉和 SS (蔗糖 synthase )的活动的核, GBSS (小粒界限淀粉 synthase ), Feiyu 3 的 SBE (淀粉分叉酶)有高淀粉内容,比 Yuyu 22 的那些显著地高,它有低淀粉内容,在 10 弹跳以后(在授粉以后的天)。相关分析显示了那 ADPGPPase (自动数据处理葡萄糖 pyrophosphorylase ) , DBE (淀粉初上舞台的人 ranching 酶) 没与积累在中间充满率,而是 SS 活动的率和核的淀粉被相关,迟了的时期显著地高度与积累充满率的率和核的淀粉被相关。GBSS 活动显著地高度与积累率的直链淀粉被相关,然而并非用充满率的核相关。SBE 活动显著地高度与积累充满率的率和核的胶淀粉被相关。它不是 ADPGPPase 和 DBE,但是 SS 是在开发玉米核的淀粉生合成的限制率的因素。GBSS 在直链淀粉累积上有重要效果,并且 SBE 在胶淀粉累积上有重要效果。
The filling rate and the starch accumulation in developing maize kernel were analyzed. The changes of enzyme activities associated with sucrose metabolism and starch biosynthesis were investigated. The purpose is to discuss the enzymatic mechanisms responsible for starch synthesis. Two types of maize cultivars (Zea mays), high starch maize (Feiyu 3) and normal maize (Yuyu 22), were grown in a corn field. The factors involved in starch synthesis were performed during the growth period. The kernel filling rate, the sucrose content, the starch accumulating rates and the activities of SS (sucrose synthase), GBSS (granule-bound starch synthase), SBE (starch branching enzyme) of Feiyu 3, which has high starch content, were significantly higher than those of Yuyu 22, which has low starch content, after 10 DAP (days after pollination). Correlation analysis indicated that ADPGPPase (ADP-glucose pyrophosphorylase) and DBE (starch debranching enzyme) were not correlated with the starch accumulating rates and the kernel filling rate, but the SS activity at the middle and late period were highly significantly correlated with the starch accumulating rates and the kernel filling rate. The GBSS activity was highly significantly correlated with the amylose accumulating rate, but not correlated with the kernel filling rate. The SBE activity was highly significantly correlated with the amylopectin accumulating rate and the kernel filling rate. It was not ADPGPPase and DBE, but SS was the rate-limiting factor of starch biosynthesis in developing maize kernels. GBSS had an important effect on amylose accumulation, and SBE had a significant effect on amylopectin accumulation.