中文摘要：

目的：利用高效液相色谱法研究了在模拟人体胃肠道环境中泽泻调酯活性成分泽泻醇类化合物23-乙酰泽泻醇B、24-乙酰泽泻醇A的稳定性。方法：采用色谱柱：Lichrospher C18（250mm×4．6mm，5μm），流动相：乙腈-水（80：20），流速：0．8mL·min^-1，检测波长：210am，柱温：30℃。结果：23-乙酰泽泻醇B、24-乙酰泽泻醇A在模拟胃酸性条件下不稳定，易发生转化。23-乙酰泽泻醇B的主要转化产物为泽泻醇A、24-乙酰泽泻醇A和-未知物。24-乙酰泽泻醇A的主要转化产物为泽泻醇A和-未知物。23-乙酰泽泻醇B、24-乙酰泽泻醇A在模拟体内肠道环境中稳定，不发生明显转化。结论：胃蛋白酶可能并非引起分解的主要因素。该结果可为中药泽泻剂型研究、泽泻醇类化合物的人血成分及调脂机制提供参考，并提示泽泻醇A可能也应作为中药泽泻的另一质量控制指标。

英文摘要：

Objective： To study the stability of alisol B 23 - acetate and alisol A 24 - acetate under simulated in vivo conditions by HPLC. Methods： The separation was performed on a Lichrospher C18 （250 mm x4, 6 mm,5 μm） and CH3CN -water （80： 20） as mobile phase with a flow rate of 0. 8 mL · min^-1. UV detection was set at 210 nm and column temperature was room temperature was 30 ℃. Results： Two compounds were unstable under the simulated stomach conditions and transformed rapidly. Alisol B 23 - acetate mainly transformed to alisol A 24 - acetate, Alisol A and the unknown compound. Alisol A 24 - acetate mainly transformed to alisol A and the unknown compound. But the two compounds were stable under the conditions of simulated the intestine pH and no transformation was found . Conclusion： Pepsin maybe not the major factors to cause the transformation. This results should be useful for studying the formulation of Alisma, the components in blood and regulating lipid metabolism of alisols, and also tip that alisol A may be as another index to the quality control of Alisma.