中文摘要：

为了阐明白屈菜红碱(Chelerythrine)对铜绿微囊藻(Microcystis aeruginosa)生长及光合系统的影响,研究了白屈菜红碱胁迫下铜绿微囊藻M.aeruginosa NIES-843生长、细胞色素含量及叶绿素光诱导荧光动力学变化。结果显示,当白屈菜红碱浓度≥10μg/L时,M.aeruginosa NIES-843的生长受到显著抑制。通过线性回归分析,白屈菜红碱对M.aeruginosa NIES-843生长50%抑制浓度(EC50)为(30.62±1.32)μg/L。当白屈菜红碱浓度为160μg/L时,M.aeruginosa NIES-843单位细胞内叶绿素a(Chl.a)和类胡萝卜素含量均显著低于对照。Chl.a光诱导荧光动力学分析结果显示,白屈菜红碱胁迫下单位反应中心吸收的光能(ABS/RC)、单位反应中心捕获的用于还原QA的能量(TR0/RC)及单位反应中心捕获的用于电子传递的能量(ET0/RC)均受到显著抑制。光合系统Ⅱ(PSⅡ)能量分配比率参数分析结果显示,白屈菜红碱能显著抑制光合系统反应中心电子供体侧电子传递。

英文摘要：

The effects of chelerythrine on the growth, pigments contents and photosynthetic system of Microcystis aeruginosa NIES-843 were studied in this paper. The results indicated that the growth of M. aeruginosa NIES-843 was inhibited by chelerythrine at the concentration of≥10μg/L. Regression analysis showed that the EC50 of chelerythrine on the growth of M. aeruginosa NIES-843 was (30.62±1.32) μg/L. Intracellular pigments of M. aeruginosa NIES-843 decreased at the concentration of 160 μg/L. Results of chlorophyll fluorescence transient analysis suggested that the absorbed energy (ABS/RC), the trapped energy for reduce of QA (TR0/RC) and the trapped energy for electron transport (ET0/RC) were all inhibited by chelerythrine. Energy fluxes ratios analysis of PSⅡindicated that the electrons provid-ing side of reaction centre was the targets of toxicity of chelerythrine on M. aeruginosa NIES-843.