中文摘要：

【目的】克隆红麻查尔酮合成酶（CHS）及其异构酶（CHI）基因并构建CHS基因的植物表达载体,为进一步研究其在红麻育性及抗逆性中的作用奠定基础。【方法】提取红麻不育系（P3A）、保持系（P3B）花药RNA和DNA,以红麻花药转录组高通量测序数据为基础,利用同源克隆技术获得CHS和CHI基因,并利用载体重组技术构建CHS基因植物表达载体。【结果】红麻CHS基因c DNA全长序列为1236 bp,包含一个1170 bp开放阅读框,编码389个氨基酸;其DNA序列全长为1329 bp,包含2个外显子和1个内含子。CHI基因c DNA序列为724 bp,最大开放阅读框为630 bp,编码209个氨基酸;其DNA序列全长为1087 bp,包含4个外显子和3个内含子。【结论】成功克隆获得红麻CHS和CHI基因的序列,构建的CHS基因植物表达载体p BI121-CHS可用于该基因功能的研究。

英文摘要：

[ Objective ]The present study was conducted to clone chalcone synthetase （CHS） and chalcone isomerase （CHI） genes in kenaf, and construct CHS plant expression vector in order to lay the foundation for further researches of their functions in kenaf ferility and stress resistance. [Method]The DNA and eDNA of kenaf male sterility （P3A） and maintainer line （P3B） anthers were amplified after extracting of DNA. The CHS and CHI genes were cloned by using homologous cloning method based on kenaf transeriptome data from high-throughput sequencing. By using carrier re- combination technology, the plant expression vector of CHS gene was constructed. [ Result ] It showed that, the eDNA full-length sequence of CHS was 1236 bp in length, contained an open reading frame （ORF） of 1170 bp, and encoded 389 amino acids. The DNA full-length sequence of CHS gene was 1329 bp in length, and contained two exons and one intron. The eDNA full-length sequence of CHI was 724 bp which contained an open reading frame （ORF） of 630 bp and encoded protein with 209 amino acids. The length of DNA sequence in CHI gene was 1087 bp in length which con- tained four exons and three introns. [ Conclusion ] The sequences of CHS and CHI genes was successfully cloned, and the constructed plant expression vector pBI121-CHS of CHS gene could be used for its functional researches.