中文摘要：

目的：研究线粒体数目与肝癌生长的相关性。方法：利用电子显微镜技术定量肝癌组织中线粒体数目,利用免疫组织化学技术评估肝癌组织中的线粒体标志物COX Ⅳ及HSP60表达水平,分析电镜肝癌线粒体定量结果与COX Ⅳ及HSP60表达量之间的相关性,并比较肝癌中线粒体数目、COX Ⅳ及HSP60表达量与肝癌直径之间的关系。结果：与癌旁相比,肝癌组织中线粒体数目（P〈0.001）、COX Ⅳ及HSP60的表达量显著降低（P=0.0417,P=0.0290）。COX Ⅳ及HSP60表达水平与线粒体数目无显著相关（r^2=0.009,P=0.5468;r^2=0.056,P=0.1396）。线粒体数目与肿瘤直径显著负相关（r^2=0.1086,P=0.0434）,COX Ⅳ及HSP60表达量与肿瘤直径无显著相关（r^2=0.0251,P=0.3287;r^2=0.0461,P=0.1830）。结论：线粒体数目是潜在的肝癌生长标志物。但常用的线粒体定量分子HSP60与COX Ⅳ并不能准确定量肝癌线粒体。

英文摘要：

Objective： To evaluate the correlation between mitochondrial number and tumor growth in Hepatocellular carcinoma （HCC）. Methods： Transmission electron microscope was used to quantify the number of mitochondria in HCC tissues. Immunohistochemistry was used to evaluate the expression levels of COX IV and HSP60 in HCC tissues. Results： Mitochondrial number （P〈0.001） and the expression levels of COX IV and HSP60 （P=0.0417, P=0.0290） were decreased in HCC tissues when compared to paired non-tumor tissues. Correlation analysis showed that mitochondrial number （r2=0.1086, P=0.0434）, but not COX IV and HSP60 （r2=0.009, P=0.5468; r2=-0.056, P=0.1396）, was significantly negatively associated with tumor diameter. No significant correlation was found between the expression level of COX 1V or HSP60 and mitochondrial number in HCC tissues （12=0.0251, P=0.3287; r2=-0.0461, P=0.1830）. Conclusions： Mitochondrial number is a potential biomarker for evaluating tumor growth and prognosis of HCC. Whereas, the commonly used molecular markers COX IV and HSP60 are not suitable for quantification ofmitochondria in HCC tissues.