中文摘要：

单链抗体已用于抗乙肝病毒（hepatitis B virus,HBV）的研究,目前已研制出作用于各种靶点,如HBV表面抗原pre-S1、核心蛋白（hepatitis B virus core antigen,HBc）、DNA聚合酶及X蛋白的多种单链抗体。单链抗体对偶联的分子具有靶向定位作用,因此,对抗原的亲和性大小、对靶细胞内化（Internalization）的强弱及其自身结构的稳定性是影响单链抗体应用的主要因素。

英文摘要：

Single-chain Fv Fragment（ScFv） has been introduced into the study of inhibiting Hepatitis B virus（HBV） replication.Nowadays,ScFvs bound to HBV pre-S1,core antigen,DNA polymerase and X protein have been developed,respectively.ScFv can make the coupled molecule with therapeutic effects localized at the target site.Thus,affinity with the antigen,internalization property and structural stability of ScFv are the main factors limiting the utilization of ScFv.