中文摘要：

目的：探讨环孢霉素A（CSA）对抗阿霉素人急性早幼粒白血病细胞系HL-60/ADM的耐药逆转作用,并比较二者之超微弱发光强度的特点.方法：选择环孢霉素A（CSA）作为耐药逆转剂,分别采用MTT法、流式细胞仪和免疫组化法分析CSA对人白血病耐药细胞系的毒性及逆转效果;用IFFM-D型流动式化学发光仪检测细胞的超微弱发光强度.结果：当CSA浓度在4ug/mL以下时,对HL-60/ADM无明显毒性作用,超过此浓度,其毒性呈剂量效应（P＜0.001）.当CSA浓度为0.5ug/mL时就有明显逆转作用,随着CSA剂量增加,逆转作用逐渐增强（P＜0.01）,当CSA剂量达8ug/mL以上时对细胞存活产生明显的影响;流式细胞仪分析细胞周期可见逆转耐药细胞系HL-60/ADM＋CSA较耐药细胞系HL-60/ADM的G2期细胞显著增多,而S期细胞明显减少（P＜0.01）;免疫组化结果显示HL-60/ADM细胞膜上P-gp高表达,经CSA作用后其表达下降;在10-4m01/L鲁米诺及0.3%双氧水条件下HL-60/ADM细胞超微弱发光强度高于用CSA逆转后的细胞（P＜0.001）.结论：CSA能有效逆转HL-60/ADM的耐药性;逆转后细胞超微弱发光强度值的降低可能与其细胞增殖速度减慢,DNA复制减少,细胞内氧化代谢减弱以及自由基水平降低等因素有关.

英文摘要：

Objective：To evaluate the reversed influence of cyclosporine A （CSA） on multidrug resistant （MDR） human leukemic cell line HL-60/ADM, and to investigate the relationship of the ultraweak photon emission （UPE） change between HL- 60/ADM cells and the reversed HL-60/ADM cells （HL-60/ADM＋CSA）.Methods：The cytotoxicity and the reversed effects of CSA on multdrug resistance of human leukemic cell IineHL-60/ADM were studied by MTT colorimetric, Flow CytoMeter （FCM） and immunochemical assays; IFFM-D chemiluminescence＇s instrument, which was made by Xi＇an REMEX Analytic Instrument Corporation, was used to detect the intensity of UPE for HL-60/ADM and its reversed cell line HL-60/ADM＋CSA. Results ： CSA less than 4 ug/ml had no significant cytotoxicity on HL-60/ADM, and the cytotoxicity rised with CSA concentration increasing; while CSA （8ug/mL） would influence the survival rate of HL-60/ADM cells; CSA （4ug/ml） combined with ADM （lug/ ml） could obviously restrain the growth of HL-60/ADM cells（P〈0.001 ）, The FCM results showed that the cell number of HL-60/ ADM＋CSA cells in G2 cycle had a sharp increase and decreased obviously in S cycle, compared with HL-60/ADM cells（P〈0.01 ）. The Pgp expression of HL-60/ADM was decreased clearly after 72 hours＇ exposure to CSA （4ug/ml） by immunochemical assays; Under the same reactve systems （10-4mol/L luminol and 0.3% H202）, also with the same volume and cells concentration, the intensity of ultraweak photon emission of HL-60/ADM cells was lower than that of HL-60/ADM＋CSA cells （P〈0.001）. Conclusion：The MDR of HL-60/ADM could be reversed by low dose of CSA effectively. The decrease of the UPE intensity in HL--60/ADM＋CSA cells might be correlated with weakening of the oxidized metabolic reaction and decrease of DNA duplication after the reversion; another possible mechanism may relate to the variation of the oxygen radicals inside the reversed cells.