对硫磷(Parathion,PT)、甲基对硫磷(Methyl—parathion,PTM)和杀螟硫磷(Fenitrothion,FT)是常见的有机磷农药,它们具有相似的分子结构,常被用作杀虫剂.可用荧光光度法、紫外-可见光度法、气相色谱法和高效液相色谱等对其进行测定.Guilbauh等采用荧光动力学法对一些有机磷和有机碳酰化合物进行测定.反应基于过硼酸钠将有机磷农药氧化为过磷酸盐或过碳酸盐阴离子,氧化产物再将无荧光的吲哚氧化成荧光物质吲哚酚的原理,但该法选择性不够好.
A kinetic-spectrophotometric method for the determination of parathion, methyl-parathion and fenitrothion was developed. It was based on the kinetic reaction of these compounds with H2O2 in alkaline medium. Under the optimal conditions, quantitative analysis for each compound in mixtures was investigated with the aid of multivariate calibration methods. The proposed method was applied to several water samples spiked with known amounts of the pesticides with satisfactory results.