中文摘要：

研究了CdTe量子点（QDs）荧光猝灭法测定盐酸四环素含量的分析方法。以3-巯基丙酸（3-MPA）修饰的CdTe QDs为荧光探针,考察了缓冲液体系及pH值、反应时间等不同条件下盐酸四环素对CdTe QDs的荧光猝灭作用。结果表明在pH 7.5的0.10mol·L^-1磷酸盐缓冲溶液（PBS）中,反应时间5min时,盐酸四环素浓度在20-180μg·mL^-1范围内呈良好的线性关系（r=0.9978）,检测限（S/N=3）为1.3μg·mL^-1,对盐酸四环素胶囊中盐酸四环素含量进行定量测定,相对标准偏差（RSD）在1.2%-2.7%之间,加标回收率在96.5%-101.1%之间。该方法快速、简便、灵敏、准确,可用于实际样品中盐酸四环素含量的测定。

英文摘要：

A method for the determination of tetracycline hydrochloride was developed by using quantum dots（QDs） as the fluorescence probe. The fluorescence quenching of tetracycline hydrochloride to CdTe QDs has been systematically investigated by using 3-MPA modified CdTe QDs as a fluorescence probe. The detection conditions such as the buffer fluid system,pH of buffer and reaction time were optimized. The fluorescence was quenched stably under PBS（ pH 7.5,0.10 mol·L^-1） ,and the response time was 5 min. The results show that tetracycline hydrochloride has a good linearity in the range of 20-180 μg· mL^-1 （r=0. 9978 ）,the relative standard deviation is between 1.2% and 2.7% and the recovery is in the range of 96.5%-101. 1%. This method is repid and sensitive and can be used in the determination of tetracycline hydrochloride from Tetracycline Hydrochloride Capsules with good repeatability.