中文摘要：

第二等的种子休眠(SSD ) 为 canola 地里的志愿者植物负责，它在 canola 生产引起一系列问题并且为对转基因的 canola 的环境安全评价用作一个重要特点。有强壮的 SSD 的 canola 栽培变种被用来在它的在第二建立卓见进种子 transcriptomes 没有由 RNA-seq 分析的休眠的休眠种子和控制种子，试图决定 SSD 的分子的生态的描述。数据集(超过 4 ? Gb ) 从每件样品有效序列被获得，它被联合 novo 集合执行 de。装配序列与长度由 314,261 碎片组成了 ?>? 100 ? bp，包括长度的 29,740 个长抄本 ?? 500 ? bp。功能的注解显示 1,641 个长抄本能被分成蛋白质(轮牙) 的 orthologous 组的 24 簇， 16,515 个抄本被连接到 2,648 基因本体论(去) 术语。有 452 个长抄本，显著地不同的表示由阀值识别了 ?>?2 褶层表示变化(P ? Arabidopsis 基因。工厂荷尔蒙 abscisic 酸和赤霉素作为种子休眠和萌芽的枢轴的管理者被知道。尽管为任何一个生合成负责或每荷尔蒙发信号的基因能广泛地从 SSD transcriptome 被验证，他们的表示证据没能与 SSD 的正式就职相关。把本体论和 KEGG orthology，以及 SSD 的候选人基因的表示模型基于基因的充实的术语，我们建议那丰满的酸新陈代谢可能在 canola 在 SSD 含有。这里报导的信息可以在转基因的 canola 在对 SSD 的环境安全评价的进一步的理解起一个重要作用。

英文摘要：

Secondary seed dormancy （SSD） is responsible for volunteer plants in canola fields, which causes a series of problems in canola production and serves as an impor- tant trait for the environmental safety assessment of transgenic canola. A canola cultivar with strong SSD was used to establish insight into seed transcriptomes in its secondarily dormant seeds and control seeds without dor- mancy by RNA-seq analysis, aiming to determine the molecular ecological characterizations of SSD. A dataset （more than 4 Gb） of valid sequences was obtained from each sample, which was combined to carry out the de novo assembling. The assembled sequences consisted of 314,261 fragments with length 〉 100 bp, including 29,740 long transcripts of length 〉 500 bp. Functional annotation indicated that 1,641 long transcripts could be categorized into 24 cluster of orthologous groups of proteins （COGs） and 16,515 transcripts were linked to 2,648 gene ontology（GO） terms. There were 452 long transcripts with signifi- cantly different expression identified by a threshold of 〉 2- fold expression change （P 〈 0.001） between samples, among which 343 transcripts were unambiguously homologous to Arabidopsis genes. The plant hormones abscisic acid and gibberellins were known as the pivotal regulators of seed dormancy and germination. Although genes responsible for either biosynthesis or signaling of each hormone could be widely verified from the SSD transcriptome, theirs expression evidences failed to correlate with the induction of SSD. Based on the enriched terms of gene ontology and KEGG orthology, as well as the expression models of candidate genes of SSD, we proposed that fatty acid metabolism might implicate in SSD in canola. The information reported here may play a significant role in further understanding of environmental safety assessment of SSD in transgenic canola.