中文摘要：

目的研究大黄素在放射增敏过程中对鼻咽癌CNE-1细胞是否产生自噬现象,并探讨其放射增敏作用与自噬相关基因mRNA表达的关系。方法鼻咽癌细胞分为空白组、大黄素组、照射组和大黄素联合射线增敏组,采用透射电镜观察各组细胞内自噬小体的形成及超微结构的改变,并运用荧光定量PCR方法检测基因ULK1、GABARAPL1、Beclin1、Bcl-2的mRNA表达水平。结果除空白组外其余各实验组均可观察到自噬小体的形成。与空白组比较,各组ULK1mRNA表达均升高（P〈0.05）,其中大黄素增敏组表达最高（P〈0.01）;而Beclin1和Bcl-2 mRNA表达下调;GABARA-PL1除大黄素增敏组升高外（P〈0.05）,其它各组变化不明显。结论大黄素在鼻咽癌细胞放射增敏过程伴随着自噬产生,自噬性死亡可能是大黄素放射增敏的途径之一。

英文摘要：

Aim To investigate whether emodin induces autophagy in the nasopharyngeal carcinoma CNE-1 cells after radiation combined with emodin and explore the relationship between the radiotherapy sensitization and autophagy-related gene mRNA expression.Methods Nasopharyngeal carcinoma CNE-1 cells were divided into control group,emodin alone group,radiation alone group and radiation combined with emodin group.After different treatments,the ultrastructural changes of autophagosome were observed by transmission electron microscope（TEM）.The mRNA expressions of ULK1,GABARAPL1,Beclin1 and Bcl-2 were detected by the Real-time PCR.Results Autophagosome or autophagolysosome was observed in all groups except in the control cells.Compared with the control group,the results of Real-time PCR showed that ULK1 mRNA expression in all the experimental groups increased（P0.05）.The radiotherapy sensitization group showed the highest（P0.01）.However,Beclin1 and Bcl-2 mRNA expression were down-regulated.GABARAPL1 expression had no remarkable changes among the experimental groups,except in radiotherapy sensitization group where it was up-regulated（P0.05）.Conclusions Emodin induces autophagy in the nasopharyngeal carcinoma CNE-1 cells after radiation combined with emodin.Autophagic cell death（ACD） may be one of the paths of the radiosensitization sensitization effect.