中文摘要：

目的探讨人参皂苷Rgl热裂解产物（HPPRgl）对H22荷瘤小鼠的抗肿瘤作用以及机制。方法建立ICR小鼠H。皮下移植瘤小鼠模型，观察HPPRgl的抗肿瘤作用。首先将H22荷瘤小鼠随机分为空白组（normal）、模型组（mod—e1）、阳性药环磷酰胺组（CTX，30mg·kg-1）、HPPRgl低、中和高给药组（10、20和40mg·kg-1）。通过计算抑瘤率、脏器指数和肿瘤组织切片染色来观察HPPRgl的抗肿瘤作用。结果与模型组比较，HPPRgl3个剂量组均能剂量依赖性抑制H22肿瘤的增长（r=0．99，P〈0．05），抑瘤率分别为35．7％，42．9％和47．3％；此外，HPPRgl能够不同程度升高小鼠血清中肿瘤坏死因子（TNF）-α，干扰素（IFN）-γ和白细胞介素（IL）-2水平，病理组织染色表明，HPPRgl能够明显促进肿瘤细胞凋亡和坏死；其主要通过抑制瘤重细胞增殖及促凋亡而发挥抗肿瘤作用。结论HPPRgl对H22移植瘤具有明显的抑制作用，其机制可能与促进肿瘤细胞凋亡及提高机体免疫力有关。

英文摘要：

OBJECTIVE To investigate the antitumor effect and molecular mechanism of ginsenoside Rgl pyrolysis products （ HPPRgl ） on H22 tumor bearing mice. METHODS To establish tumor model of transplanting H22 tumor-bearing mice and observe the anti-tumor effects of HPPRgl, H22 tumor-bearing mice were randomly divided into groups of control, model, cyelophosphamide （CTX, 30 mg kg-1 ）, low dosage of HPPRgl （HPPRgl-L, 10 mg kg-1 ）, middle dosage of HPPRgl （HPPRgl-H, 20 mg kg-1 ） and high dosage of HPPRgl （ HPPRgl-H, 40 mg kg - 1 ） groups, respectively. Through evaluating inhibition rates of tumors, organ indiees, and levels of TNF-α, IFN-γ and IL-2 to observe the anti-tumor effect of HPPRgl. In addition, H＆E and Hoechst 33258 strai- ning were used to observe the apoptosis of H22 tumor cell. RESULTS Compared with the model group, the three dose groups of HP- PRgl can inhibit tumor proliferation. Mainly through the inhibition of tumor cell proliferation and pro-apoptosis to exert anti-tumor effect. CONCLUSION HPPRgl has a significantly inhibitory effect on H22 tumor-bearing mice, the mechanism may related to pro- mote apoptosis of tumor cells and improve immunity.