中文摘要：

以表达Cry1Ab杀虫蛋白的两种转Bt基因抗虫玉米MON810和Bt11为材料，采用ELISA方法研究了玉米散粉时沉积在植株叶腋处Bt玉米花粉中Cry1Ab杀虫蛋白的田间降解动态，比较了两种Bt玉米花粉中Cry1Ab杀虫蛋白的降解速度．结果表明，Bt玉米花粉中Cry1Ab杀虫蛋白是逐渐降解的，且降解速度逐渐加快，但两种Bt玉米花粉中Cry1Ab杀虫蛋白降解速度不同，MON810中的Bt杀虫蛋白降解较快，而Bt11中的降解较慢，d15时在MON810叶腋处的花粉中已经检测不到Bt杀虫蛋白，而在Bt11花粉中Bt杀虫蛋白到d18才完全降解，两种Bt玉米花粉中杀虫蛋白的初始含量差异显著，且在各个取样时间内Bt杀虫蛋白的残留量存在显著差异，玉米散粉时留在田间的Bt玉米花粉中的Cry1Ab杀虫蛋白不会在自然界累积，图1表1参34

英文摘要：

The field degradation dynamics of CrylAb insecticidal protein in transgenic Bacillus thuringiensis corn pollen of two Bt corn hybrids of events MON810 and Bt11 accumulated in the leaf axile at pollen-shedding stage were investigated and compared. The levels of CrylAb protein expression in pollen of the two transgenic Bt corns were quantified by using enzyme-linked immunosorbent essay （ELISA）. The results showed that the CrylAb toxin in Bt corn pollen was gradually and rapidly degraded. The degradation speeds were significantly different between the two events. The CrylAb insecticidal protein in corn pollen of event Btll was slower than that of event MON810. No CrylAb insecticidal protein was detected in the pollens of events MON810 and Btll in 15 d and 18 d, respectively. The initial concentrations of CrylAb and also within the same sampling time for detection were significantly different between the two events. The Cryl Ab insecticidal protein within transgenic Bt corn pollen in the leaf axiles of events MON810 and Btll could not be accumulated in cornfield. Fig 1, Tab 1, Ref 34